Supplied as a lyophilized powder containing human albumin, mannitol and NaCl.
The principle active ingredient is the low molecular weight form of urokinase, and consists of an A chain of 2,000 daltons linked by a sulfhydyl bond to a B chain of 30,400 daltons.
Application
Urokinase from human kidney cells has been used as a component of digestion mix for three-dimensional fibrin culture. It has also been used in plasminogen activation assay.
Biochem/physiol Actions
Urokinase was shown to augment proliferation of renal tumor cells.
Urokinase is a protease that cleaves plasminogen to form plasmin. It binds to cell surface receptors (uPAR) from which it regulates cell adhesion and migration during tissue remodeling and which also activate intracellular signaling pathways. Urokinase is composed of three domains: the amino terminal domain which is homologous to epidermal growth factor is attached through a central kringle domain to the protease domain in the carboxyl terminus.
Other Notes
Note: Higher activities are obtained when human plasminogen is used as substrate.
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