V900830

DL-Dithiothreitol

Vetec^™, reagent grade, 98%

• Synonim(y): threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
• Wzór liniowy: HSCH₂CH(OH)CH(OH)CH₂SH
• Numer CAS: 3483-12-3
• Masa cząsteczkowa: 154.25
• EC Number: 222-468-7
• UNSPSC Code: 12352209
• PubChem Substance ID: 329830191
• Beilstein/REAXYS Number: 1719757
• MDL number: MFCD00004877

Właściwości

• grade: reagent grade
• product line: Vetec^™
• assay: 98%
• mp: 41-44 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
• InChI key: VHJLVAABSRFDPM-QWWZWVQMSA-N

Opis

Application

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05,GHS07
• Słowo ostrzegawcze: Danger
• Kody zagrożeń: H302,H314,H412
• Ostrzeżenia: P260 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
• Klasa składowania: 8A - Combustible corrosive hazardous materials