CC130

Human Vitronectin

Name: Human Vitronectin
Brand: MM
Price: 179 SGD
Availability: InStock

recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM^®

别名:

S-protein, Serum-spreading factor, V75

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选择尺寸

500 μG

$179.00

预计发货时间2025年12月24日详情

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关于此项目

分類程式碼代碼:

12352202

eCl@ss:

32160405

NACRES:

NA.75

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产品名称

PluriSTEM-XF^® 重组玻连蛋白, Human pluripotent stem cell culture.

生物源

Escherichia coli
human

品質等級

100

化驗

≥92% (SDS-PAGE)

形狀

liquid

製造商／商標名

PluriSTEM^®

濃度

0.5 mg/mL

技術

cell attachment: suitable
cell culture | mammalian: suitable
cell culture | stem cell: suitable

輸入

sample type: human embryonic stem cell(s)

UniProt登錄號

P04004

運輸包裝

dry ice

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此商品	V0132	V8379	5051
Sigma-Aldrich CC130 PluriSTEM-XF^® 重组玻连蛋白	Sigma-Aldrich V0132 Vitronectin from rat plasma	Sigma-Aldrich V8379 玻连蛋白来源于人类血浆	Sigma-Aldrich 5051 玻连蛋白
biological source Escherichia coli, human	biological source rat plasma	biological source human plasma	biological source human plasma
technique(s) cell attachment: suitable, cell culture \| stem cell: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
assay ≥92% (SDS-PAGE)	assay -	assay -	assay >95%
concentration 0.5 mg/mL	concentration -	concentration -	concentration 0.5 mg/mL
Quality Level 100	Quality Level 200	Quality Level 300	Quality Level 100
form liquid	form lyophilized powder	form lyophilized powder	form solution

一般說明

PluriSTEM 重组玻连蛋白是matrigel的有效无异种替代品，已被验证可以使用PluriSTEM人ES/iPS培养基（Millipore目录编号SCM130，SCM132），E8和NutriStem人ES细胞生长培养基成功培养人ES和iPS细胞。

PluriSTEM重组玻连蛋白是人玻连蛋白（基因20-398aa片段）的截短形式，其通过密码子优化构建，在大肠埃希菌中表达并纯化为包含体。使用独特的“温度变化包涵体重折叠”技术将最终产物重折叠并进行色谱纯化。最终产品被认为是无异种的。

應用

支持人es/ips细胞的无饲养层和无血清生长，同时维持无异种培养条件。

研究类别
干细胞研究

细胞结构

ECM & 粘连

外觀

在含有专有处方NaCl、KCl、EDTA、精氨酸、DTT和甘油的20 mM pH值8.0 Tris-HCl缓冲液中对产品进行无菌过滤。

準備報告

储存于-20°C，直到小瓶上的有效期为止。解冻后，产品在2-8°C下可稳定储存两周。解冻后的玻连蛋白可分装于-20°C保存。应避免其他冻融循环。

分析報告

在每孔1 mL人ESC培养基（6孔板）中使用5-10 μg重组玻连蛋白培养的人ES细胞（H1）上测试每个批次。

法律資訊

PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers.

Mutsumi Kobayashi et al.

Stem cell reports, 17(3), 507-521 (2022-02-13)

In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which

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