生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
528, monoclonal
形狀
liquid
包含
≤0.1% sodium azide as preservative
物種活性
human
應無反應活性
mouse, rat
製造商／商標名
Calbiochem®
儲存條件
do not freeze
dilution
(Flow Cytometry (5 µg/mL)
Frozen Sections (2.5 µg/mL)
Immunofluorescence (5 µg/mL)
Immunoprecipitation(1 µg/sample)
Neutralization Studies
Paraffin Sections )
同型
IgG2a
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egf(25313)
一般說明
Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
Recognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.
應用
Flow Cytometry (5 µg/ml)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation(1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
Frozen Sections (2.5 µg/ml)
Immunofluorescence (5 µg/ml)
Immunoprecipitation(1 µg/sample)
Neutralization Studies (see comments and application references)
Paraffin Sections (see application references)
包裝
Please refer to vial label for lot-specific concentration.
分析報告
Negative Control
HL-60 cells or human brain
HL-60 cells or human brain
Positive Control
A-431 or normal skin tissue
A-431 or normal skin tissue
其他說明
Hunter T. 1984. Nature311, 414.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
Masui, H., et al. 1984. Cancer Res.44, 1002.
Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
Ushiro, H. and Cohen, S. 1980. J. Biol. Chem.255, 8363.
This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Toxicity: Standard Handling (A)
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Rozanne Lee et al.
Journal of biomolecular screening, 13(3), 210-217 (2008-03-04)
Monoclonal antibodies (mAb) are not only useful reagents but also represent a promising type of therapeutics due to their high affinity and exquisite specificity for their antigens. A critical step in mAb generation is to identify antigen-specific antibodies. Although enzyme-linked
Adam C Midgley et al.
The Journal of biological chemistry, 288(21), 14824-14838 (2013-04-17)
Fibroblast to myofibroblast differentiation drives effective wound healing and is largely regulated by the cytokine transforming growth factor-β1 (TGF-β1). Myofibroblasts express α-smooth muscle actin and are present in granulation tissue, where they are responsible for wound contraction. Our previous studies
David Cachia et al.
Journal of neuro-oncology, 125(2), 401-410 (2015-09-12)
Gliosarcoma is classified by the World Health Organization as a variant of glioblastoma. These tumors exhibit biphasic histologic and immunophenotypic features, reflecting both glial and mesenchymal differentiation. Gliosarcomas can be further classified into primary (de novo) tumors, and secondary gliosarcomas
F Terzi et al.
The Journal of clinical investigation, 106(2), 225-234 (2000-07-21)
The role of EGF in the evolution of renal lesions after injury is still controversial. To determine whether the EGF expression is beneficial or detrimental, we generated transgenic mice expressing a COOH-terminal-truncated EGF-R under the control of the kidney-specific type
Inmaculada Bañón-Rodríguez et al.
The EMBO journal, 33(2), 129-145 (2014-01-15)
Establishing the correct orientation of the mitotic spindle is an essential step in epithelial cell division in order to ensure that epithelial tubules form correctly during organ development and regeneration. While recent findings have identified some of the molecular mechanisms
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