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Merck

MR-106

EmbryoMax® KSOM小鼠胚胎培养基

(1X), Liquid, with 1/2 Amino Acids

别名:

K+ Simplex Optimised Medium (KSOM), KSOM Mouse Embryo Media

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关于此项目

分類程式碼代碼:
12352207
eCl@ss:
32160801
NACRES:
NA.71

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品質等級

300

形狀

liquid

製造商／商標名

Specialty Media
EmbryoMax®

技術

cell culture | embryo: suitable
cell culture | stem cell: suitable

輸入

sample type: mouse embryo(s)

相关类别

應用

EmbryoMax® KSOM小鼠胚胎培养基已用于培养小鼠胚胎的胚胎发育。[1][2]

準備報告

可以在-20°C下保存直至瓶上的有效期。


解冻后应在2周内使用。

法律資訊

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Benjamin L Kidder
Methods in molecular biology (Clifton, N.J.), 1150, 191-199 (2014-04-20)
Epigenetic regulation of gene expression in the germline is important for reproductive success of mammals. Misregulation of genes whose expression is correlated with reproductive success may result in subfertility or infertility. To study epigenetic events that occur during oocyte maturation
Yilong Miao et al.
STAR protocols, 2(1), 100298-100298 (2021-02-04)
The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide

商品

胚胎培养基

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications

Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Questions

1–5 of 5 Questions  

  1. Could you please send me the medium composition? We need to know the concentration of BSA, essential amino acids and non-essential amino acids, please

    1 answer

    1. This medium is listed as a 1/2 amino acid formulation. The complete list of components as well as the concentration of each are listed below:
      Essential and Non-essential amino acids are also designated.
      MR-106-D EmbryoMax KSOM Mouse Embryo Medium
      Component - mg/L – E = Essential, N = Non-Essential
      CaCl2-2H2O 250.00
      KCL 186.38
      KH2PO4 47.99
      MgSO4 7H2O 49.30
      NaCl 5551.80
      NaHCO3 2100.25
      EDTA 3.72
      D-Glucose 36.03
      Sodium Lactate 1121.00
      Lactate Na Salt 1.42 (ml)
      Sodium Pyruvate 22.00
      Bovine Serum Albumin 1000.00
      L-Arginine 63.20 E Conditionally essential
      L-Cystine 12.02 N Conditionally essential
      L-Glutamine 146.15 N Conditionally essential
      Glycine 3.75 N Conditionally essential
      L-Histidine·HCl·H2O 20.96 E
      L-Isoleucine 26.23 E
      L-Leucine 26.24 E
      L-Lysine·HCl 36.52 E
      L-Methionine 7.46 E
      L-Phenylalanine 16.52 E
      L-Serine 5.26 N Conditionally essential
      L-Threonine 23.82 E
      L-Tryptophan 5.11 E
      L-Tyrosine 18.12 N Conditionally essential
      L-Valine 23.42 E
      L-Alanine 4.45 N
      L-Asparagine·H2O 7.50 N
      L-Aspartic Acid 6.66 N
      L-Glutamic Acid 7.36 N
      L-Proline 5.76 N
      Pen G Na Salt 100,000.00 (units)
      Strep Sulfate 50.00

      Helpful?

  2. Hello, I have been working with the KSOM MR-106-D for some time and I would need to know the exact composition of all the components of this medium, could you please provide it to me. Thank you

    1 answer

    1. Please see the image below for the full formulation of this product:

      Helpful?

  3. Hi, Does it have any BSA in the medium? if so, what concentration?

    1 answer

    1. This medium contains Bovine Serum Albumin at 1000 mg/L.

      Helpful?

  4. Hi, can I know the composition of the media? Namely glucose, lactate and pyruvate is of best interest! Thanks

    1 answer

    1. Product MR-106 has the following composition:
      D-Glucose: 36.03 mg/L
      Sodium Lactate: 1121.00 mg/L
      Sodium Pyruvate: 22.00 mg/L

      Helpful?

  5. Hello, is it possible to send the KSOM just chilled and not frozen? We would prefer to get the media only on ice to make smaller aliquots so that we freeze it for the first time ourselves. 50ml is significant volume which we are not able to use in 2 weeks

    1 answer

    1. This product is shipped in dry ice at-20°C. Items requiring special storage conditions are stored according to the requirements indicated on the product-specific website or on the product label. Please read through Storage and Transport Conditions located on the "Quality &Regulatory" page of the website: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/232/426/storage-transport-conditions-ms.pdf

      Helpful?

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