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Merck

SCT037

Sigma-Aldrich

BioTracker Far-red Labile Fe2+染料

Live Cell imaging probe for labile ferrous (Fe2+) ions, localizing inside endoplasmic reticulum (ER). Also suitable for flow cytometry.

别名:

ER and golgi Fe2+ probe, cellular iron II ion probe

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分類程式碼代碼:
12352207
NACRES:
NA.47

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形狀

lyophilized (solid blue)

應用

cell analysis

檢測方法

fluorometric

相关类别

一般說明

铁是人体中最丰富的过渡金属，参与许多重要的生物学过程，如呼吸、氧气运输和与氧气合作产生能量。在活细胞中，铁主要以亚铁(Fe2+)和铁(Fe3+)离子形式存在。作为细胞氧化损伤的主要贡献者，Fe2+与癌症和神经退行性疾病等严重疾病有关，因为它能通过与氧、超氧化物和过氧化氢接触产生有害的活性氧(H2O2)。BioTracker远红不稳定Fe2+染料是一种专门检测不稳定铁离子(Fe2+)的荧光探针。该探针可以选择性地与Fe2+分别与其他金属离子反应，不可逆地转化为远红色荧光物质。它主要定位于内质网(ER)和/或高尔基体内。它还可以与配备红色激光的流式细胞仪一起使用。FerroFarRed在浓度高达100 μM（常用浓度的约20倍）时几乎没有细胞毒性。该产品与Goryo Chemical, Inc.的FerroFarRedTM (GC903-01)相同。光谱特性：吸光度（最大值）： 646nm发射（最大值）：662nm（远红外）研究类别：活细胞染料

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儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Questions

1–4 of 4 Questions  

  1. What is the solubility limit of this compound in DMSO? Is it possible to make a stock solution of a higher concentration than 1 mM in DMSO to use the compound at higher working solution concentrations than 5 µM while keeping the DMSO concentration low?

    1 answer

    1. As per established protocol, add 50 µL of DMSO to one vial (containing 50 nmol) to prepare a 1 mM stock solution. Preparing stock solutions at concentrations higher than 1 mM in DMSO is not recommended. Adhering to the protocol ensures consistency and stability of the compound.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/202/058/sct037ds.pdf

      Helpful?

  2. does this reagent stain Fe2+ localized only inside endoplasmic reticulum (ER)?

    1 answer

    1. This item localizes primarily, but not exclusively, inside the endoplasmic reticulum (ER). This compound may also localize in the Golgi. While not confirmed, there is some indication that the material may detect Fe2+ in the cytoplasmic pool or other organelles.

      Helpful?

  3. Does the BioTracker FAr-red Labile Fe2+ Dye stain dead or non-viable tissue, or does it exclusively stain live cells as advertised?

    1 answer

    1. SCT037, BioTracker Far Red Labile Fe2+ Dye exclusively stains live or viable cells and does not have a staining protocol for use on dead or non-viable cells. SCT037 is equivalent to FerroFarRed™ from Goryo Chemical.

      Helpful?

  4. Hi, I was wondering if the BioTracker Labile Iron dye # SCT037 can be freeze-thawed after being resuspended in DMSO for a second use. Also, is it fixable? Thank you.

    1 answer

    1. Storing as a solution is not recommended. In solution, the reagent degrades and the fluorescence background signal may increase.
      Yes, the FerroFarRed can be fixed under mild conditions such as 3% Paraformaldehyde for 10min at 4 °C. The fluorescence of this dye significantly diminishes when fixed in 3% PFA for 20min at RT.

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