It is not possible to have cells that are PI positive without the cells also being positive for Annexin V binding. Cells are PI positive because the membrane has been compromised. If this is the case, Annexin V can also enter the cell and bind to the PS on the internal cell membrane. The gating on the histogram for the FITC channel should be changed so that all cells that are PI positive are also Annexin V positive.
usage
(20 tests)
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Quality Level
1 of 4
此商品 | APOAC | FITC1 | SBR00028 |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) - | technique(s) - |
| usage (20 tests) | usage sufficient for 200 tests | usage - | usage - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of (Kit contains reagents for 5 conjugations.) | packaging - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| detection method fluorometric | detection method fluorometric | detection method - | detection method - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in dry ice |
Application
Features and Benefits
相比基于DNA的检测法（比如TUNEL法），可检测到细胞凋亡的更早期进程。
- 快速标记细胞。细胞染色仅需10分钟。
- 细胞无需固定或预处理，节省检测时间并满足细胞用于进一步研究。
- 试剂盒内含碘化丙啶二级染料，用于从活细胞和坏死细胞中区分出凋亡细胞。
General description
Annexin V-FITC试剂盒可以荧光检测与annexin V结合的凋亡细胞，并使用流式细胞仪进行定量。AnnexinV-FITC试剂盒使用荧光素异硫氰酸酯（FITC）偶联的annexin V标记细胞膜表面的磷脂酰丝氨酸位点。该试剂盒包含碘化丙啶(PI)，用于标记细胞膜完全破坏的坏死细胞内的DNA。这种组合可以区分早期凋亡细胞（annexin V阳性, PI阴性），坏死细胞（annexin V阳性, PI阳性），以及活细胞（annexin V阴性, PI阴性）。
Other Notes
使用前使所有组分达到室温。
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说明
价格
存储类别
10 - Combustible liquids
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When using Product APOAF, Annexin V-FITC Apoptosis Detection Kit, I have cells that are Annexin V FITC negative and PI positive. What are these cells?
1 answer-
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Can I use Product APOAF, Annexin V-FITC Apoptosis Detection Kit, to differentiate cells that are dead due to necrosis or apoptosis?
1 answer-
When using a kinetic study (various time points), you can show the progression of the cells from viable (annexin V FITC negative, PI negative), to annexin FITC positive, PI negative (membrane flip) to annexin V FITC positive, PI positive (dead). If there are cells that are double positive when starting, it is not possible to guarantee that the cell death occurred due to apoptosis with this assay.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product APOAF, Annexin V-FITC Apoptosis Detection Kit be used on fixed cells?
1 answer-
No. Product APOAF, Annexin V-FITC Apoptosis Detection Kit must be performed on live cells in order to measure Apoptosis. The assay is based on the externization of phosphatidylserine from the inner cell membrane to the outer cell membrane. If the membrane is preturbed due to fixation, non-speciifc staining of the inner cell membrane might occur
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When using the Annexin V-FITC Apoptosis Detection Kit, Product APOAF, can I use any buffer for resuspending my cells?
1 answer-
The binding buffer included in the kit needs to be used for resuspending cells. The buffer contains calcium chloride at a final (1X) concentration of 2.5 mM which is necessary for the binding of annexin V to phosphatidylserine.
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What wavelength do I use to detect Annexin V-FITC and Propidium Iodide when using Annexin V-FITC Apoptosis Detection Kit, Product APOAF?
1 answer-
Annexin V FITC will have a maximum emission of 528 nm. This can be measured in the standard FITC Channel on a flow cytometer (FL1). Propidium Iodide has a maximum emission of 620 nm. This is measured on the short red channel on a flow cytometer (FL2 or FL3).
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