Benzonase Nuclease is extremely stable. Even with extended incubations at 37 °C, Benzonase® Nuclease maintained > 90% activity for several months.
E8263
Benzonase®核酸酶，超纯
≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Benzonase® Nuclease, ultrapure
1 of 1 reviewers received a sample product or took part in a promotion
别名:
内切核酸酶 来源于粘质沙雷氏菌
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产品名称
Benzonase®核酸酶，超纯, ≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
biological source
Serratia marcescens
recombinant
expressed in E. coli
grade
ultrapure grade
assay
≥99% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
30 kDa
concentration
≥250 units/μL
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Quality Level
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此商品 | T4330 | 71206 | 70664 |
|---|---|---|---|
| grade ultrapure grade | grade - | grade - | grade - |
| biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens | biological source Serratia marcescens |
| assay ≥99% (SDS-PAGE) | assay - | assay >99% (SDS-PAGE) | assay >99% (SDS-PAGE) |
| form buffered aqueous glycerol solution | form liquid | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution |
| concentration ≥250 units/μL | concentration ≥200,000 units/mL | concentration ≥250 units/μL | concentration 25-29 units/μL |
| application(s) research use | application(s) life science and biopharma | application(s) research use | application(s) research use |
Application
用于去除蛋白样品中的核酸。
该酶已用于一项检测不受Benzonase核酸酶降解的Tf1 RNA量的检测中。粟酒裂殖酵母的Tf1元件是含有长末端重复序列的反转录转座子，可编码功能性蛋白酶、逆转录酶和整合酶蛋白。[1]它还用于从细胞裂解物中获得的核颗粒和核提取物的分馏。[2]
超纯的Benzonase核酸酶，或来自粘质沙雷氏菌的内切核酸酶，已用于一项评估十二烷基苯磺酸钠和消泡剂对粘质沙雷氏菌内切核酸酶活性影响的研究中。来自粘质沙雷氏菌的内切核酸酶已用于一项探究染色质亚基结构的研究中。
Biochem/physiol Actions
Benzonase®是一种来自粘质沙雷氏菌的基因工程改造内切核酸酶。[3][4]该蛋白质是30kDa亚基的二聚体，带有两个必需的二硫键。[5][6][7][8]该内切核酸酶可攻击并降解所有形式的DNA和RNA（单链的、双链的、线性的和环化的），并可在一系列宽泛的操作条件下具有高效性。它可将核酸完全消化为长度为3至5个碱基的5′-单磷酸末端寡核苷酸。这是从重组蛋白中去除核酸的理想选择。它还可用于需要完全消化核酸的应用。它还可降低蛋白提取物的粘度并防止细胞成团。用这种酶对蛋白样品进行预处理可通过去除任何结合的核酸而提高它在2D凝胶电泳中的分辨率。最适的酶活pH为8.0-9.2。
消化天然或热变性的DNA和RNA。
Legal Information
Benzonase®核酸酶由德国达姆施塔特默克公司和/或其附属公司提供。
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
在pH 8.0，37 °C条件下，一个单位的酶在30分钟内可将超声的鲑鱼精子DNA消化成等同于1.0 ΔA260的酸可溶寡核苷酸（2.625 ml反应体积）。
Physical form
在含有20 mM Tris HCl, pH 8.0, 2 mM MgCl2及20 mM NaCl的50%甘油中的溶液。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
Syed Nabeel-Shah et al.
Scientific reports, 10(1), 168-168 (2020-01-15)
Chromatin organization influences most aspects of gene expression regulation. The linker histone H1, along with the core histones, is a key component of eukaryotic chromatin. Despite its critical roles in chromatin structure and function and gene regulation, studies regarding the
Laure Teysset et al.
Journal of virology, 77(9), 5451-5463 (2003-04-15)
The Tf1 element of Schizosaccharomyces pombe is a long terminal repeat-containing retrotransposon that encodes functional protease, reverse transcriptase, and integrase proteins. Although these proteins are known to be necessary for protein processing, reverse transcription, and integration, respectively, the function of
P Friedhoff et al.
Protein expression and purification, 5(1), 37-43 (1994-02-01)
Overproduction of the extracellular Serratia marcescens nuclease in Escherichia coli results in aggregation and sequestration of a large amount of the protein in inclusion bodies. Only a relatively small amount is secreted into the medium from which it can be
Jyoti Garg et al.
Current biology : CB, 29(14), 2371-2379 (2019-07-10)
Mediator is a large protein complex required for basal and regulated expression of most RNA polymerase II (RNAP II)-transcribed genes, in part due to its interaction with and phosphorylation of the conserved C-terminal domain (CTD) of Rpb1 [1, 2]. Mediator
Marin Ming et al.
Vaccine, 37(22), 2960-2966 (2019-04-24)
Potency assays for vaccine products are an important regulatory requirement, and are used to assess product quality and consistency prior to lot release for clinical testing. Ideally they measure an established correlate of efficacy or protection. In cases where there
商品
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
本页列出了有关 Benzonase® 核酸酶的九个常见问题及其解答。
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
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Hello, I purchased it recently while the mailroom didn't notify me immediately when it arrived, so it stayed in 4 degree instead or -20 for around two days. Could you please provide me some information if it will highly affect the efficiency of the enzyme
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Does this benzonase contain any epitope tag?
1 answer-
Product E8263 does not have any epitope tag.
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