SBVT04
SB-MRP1-Sf9
membrane preparation for Vesicular Transport Assays, recombinant, expressed in baculovirus infected Sf9 cells
别名:
ABCC1, MRP1, MRP1 humanv membrnae vesicles, Multidrug resistance-associated protein 1
跳至
重組細胞
expressed in baculovirus infected Sf9 cells
形狀
liquid
濃度
5 mg/mL
顏色
off-white
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... ABCC1(4363)
1 of 4
此商品 | SBAT02 | SBVT06 | SBVT05 |
|---|---|---|---|
| recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells | recombinant expressed in baculovirus infected Sf9 cells |
| concentration 5 mg/mL | concentration 5 mg/mL | concentration 5 mg/mL | concentration 5 mg/mL |
| form liquid | form liquid | form liquid | form liquid |
| Gene Information human ... ABCC1(4363) | Gene Information human ... ABCC1(4363) | Gene Information human ... ABCC3(8714) | Gene Information human ... ABCC2(1244) |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
一般說明
Membrane Preparations for Vesicular Transport Assays (VT) are suitable for general drug-efflux transporter interaction studies. Both substrate and inhibitor interactions can be assessed using vesicles. The success of substrate interaction studies strongly depends on the passive permeability of the compound. High permeability substrates might not be detected. Control Membranes with no-, or significantly lower transporter activity are also available.
應用
In the vesicular transport assay so-called "inside-out" membrane vesicles containing ABC transporters are applied. Incubating substrates of the respective efflux transporter in the presence of the inverted membrane vesicles and ATP will allow measuring accumulation of the substrates into the vesicles. In many cases radiolabeled reporter substrates are used but recently SOLVO developed the new PREDIVEZTM Vesicular Transport Kits that use fluorescent reporter substrates.
The standard vesicular transport assay is an inhibitory assay performed with cold test articles. This assay provides information on any interaction between the ABC transporter and the test article. The transport of the reporter substrate is measured in the presence of the test article (typically in 7 concentrations) and IC50 is defined as the concentration inhibiting the transport of the reporter substrate by 50%.
Should radiolabeled form of the investigated compound or adequate analytical methods (LC/MS, HPLC) be available, the vesicular transport assay may be performed in a direct format without the reporter substrate and may identify substrate nature of the test article. The vesicular transport substrate assay is a low throughput assay. It is suitable for low permeability test compounds as high permeability compounds may escape from the vesicles through the lipid bilayer.
PREDIVEZ vesicular transport kit is sold separately. This transporter membrane vesicle assay requires the PREDIVEZ vesicular transport kit and corresponding control membrane below:
SB PREDIVEZ Reagent Kit for MRP1 part number SBPVR2-9RXN and corresponding control membrane SB-beta-gal-Sf9-CTRL part number SBCT03-1EA
The standard vesicular transport assay is an inhibitory assay performed with cold test articles. This assay provides information on any interaction between the ABC transporter and the test article. The transport of the reporter substrate is measured in the presence of the test article (typically in 7 concentrations) and IC50 is defined as the concentration inhibiting the transport of the reporter substrate by 50%.
Should radiolabeled form of the investigated compound or adequate analytical methods (LC/MS, HPLC) be available, the vesicular transport assay may be performed in a direct format without the reporter substrate and may identify substrate nature of the test article. The vesicular transport substrate assay is a low throughput assay. It is suitable for low permeability test compounds as high permeability compounds may escape from the vesicles through the lipid bilayer.
PREDIVEZ vesicular transport kit is sold separately. This transporter membrane vesicle assay requires the PREDIVEZ vesicular transport kit and corresponding control membrane below:
SB PREDIVEZ Reagent Kit for MRP1 part number SBPVR2-9RXN and corresponding control membrane SB-beta-gal-Sf9-CTRL part number SBCT03-1EA
外觀
Supplied as frozen membrane vesicles, containing 5 mg/ml membrane protein, labeled with volume, catalog number (transporter) and date of production.
法律資訊
Distributed for SOLVO Biotechnology, Inc.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Roger G Deeley et al.
Physiological reviews, 86(3), 849-899 (2006-07-04)
Multidrug Resistance Proteins (MRPs), together with the cystic fibrosis conductance regulator (CFTR/ABCC7) and the sulfonylurea receptors (SUR1/ABCC8 and SUR2/ABCC9) comprise the 13 members of the human "C" branch of the ATP binding cassette (ABC) superfamily. All C branch proteins share
Cornelius F H Mueller et al.
Circulation research, 97(7), 637-644 (2005-08-27)
Glutathione (GSH) is the major source of intracellular sulfhydryl groups. Oxidized GSH (GSSG) can be recycled to GSH by the GSH reductase or exported from the cell. The mechanism by which GSSG is exported and the consequence of its export
J Wijnholds et al.
Nature medicine, 3(11), 1275-1279 (1997-11-14)
The multidrug resistance-associated protein (MRP) mediates the cellular excretion of many drugs, glutathione S-conjugates (GS-X) of lipophilic xenobiotics and endogenous cysteinyl leukotrienes. Increased MRP levels in tumor cells can cause multidrug resistance (MDR) by decreasing the intracellular drug concentration. The
S-M He et al.
Current medicinal chemistry, 18(3), 439-481 (2010-12-15)
Multidrug ABC transporters such as P-glycoprotein (P-gp/MDR1/ABCB1) and multidrug resistance protein 1 (MRP1/ABCC1) play an important role in the extrusion of drugs from the cell and their overexpression can be a cause of failure of anticancer and antimicrobial chemotherapy. Recently
J H Hooijberg et al.
FEBS letters, 469(1), 47-51 (2000-03-10)
The transport mechanism by which the multidrug resistance protein 1 (MRP1) effluxes cytotoxic agents out of cells is still not completely understood. However, the cellular antioxidant glutathione (GSH) has been shown to have an important role in MRP1-mediated drug transport.
Active Filters
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持