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Merck

11024AST

Astec® CHIROBIOTIC® V Chiral (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

别名:

V手性固定相色谱柱

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关于此项目

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

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产品名称

Astec® CHIROBIOTIC® V 手性 HPLC 色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

agency

suitable for USP L88

description

HPLC Column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

matrix

High-purity silica gel particle platform
fully porous particle

matrix active group

vancomycin phase

particle size

5 μm

pore size

100 Å

operating pH range

3.5-7.0

separation technique

chiral

Quality Level

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11018AST11020AST11022AST
particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

separation technique

chiral

separation technique

chiral

separation technique

chiral

separation technique

chiral

matrix active group

vancomycin phase

matrix active group

vancomycin phase

matrix active group

vancomycin phase

matrix active group

vancomycin phase

L × I.D.

25 cm × 4.6 mm

L × I.D.

10 cm × 2.1 mm

L × I.D.

25 cm × 2.1 mm

L × I.D.

10 cm × 4.6 mm

matrix

High-purity silica gel particle platform, fully porous particle

matrix

High-purity silica gel particle platform, fully porous particle

matrix

High-purity silica gel particle platform, fully porous particle

matrix

High-purity silica gel particle platform, fully porous particle

product line

Astec®

product line

Astec®

product line

Astec®

product line

Astec®

Application

Astec® CHIROBIOTIC® V手性HPLC色谱柱可用于通过填充柱超临界流体色谱法(pSFC)分离不同的外消旋化合物(ß-封闭剂、 ß-激动剂、苯二酚类、非甾体抗炎药、巴比妥酸盐、游离氨基酸和衍生氨基酸。[1]还可用于手性液相色谱法分离人血浆中西酞普兰对映体及其两种N-去甲基代谢物和内标物阿普洛尔。[2]此外,其还可用于手性液相色谱分析酮洛芬和氟比洛芬。[3]

General description

中性分子、酰胺、酸、酯和胺对这些基于万古霉素的CSP具有相当大的对映选择性。在极性离子模式下,在CHIROBIOTIC® V上可分离多种仲胺和叔胺。CHIROBIOTIC® V已被证明具有许多蛋白质固定相的分离特性,但又具有优异的稳定性和更高的样品容量。部分尚无采用其他手性固定相分离报道的手性分析物也已在其上得到分离。CHIROBIOTIC® V和V2的成键化学特性不同,载体颗粒的孔径不同,因而各自具有不同的灵敏度和制备能力。

  • 键合相:万古霉素
  • 工作pH范围: 3.5 - 7.0
  • 粒径:5、10或16 μm
  • 孔径:100 Å (CHIROBIOTIC® V)或200 Å (CHIROBIOTIC® V2)

Other Notes

探索最适于 HPLCLC-MS分析的LiChropur试剂

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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F Péhourcq et al.
Biomedical chromatography : BMC, 15(3), 217-222 (2001-06-08)
Vancomycin is an amphoteric, glycopeptide, macrocyclic antibiotic. When attached to 5 microspherical silica gel, vancomycin proved to be an effective chromatographic chiral stationary phase that could be used in the reversed-phase mode. In this study, a bonded vancomycin chiral stationary
Chiral packed column subcritical fluid chromatography on polysaccharide and macrocyclic antibiotic chiral stationary phases.
Andrei M
Journal of Chromatography A, 785 (1), 159-171 (1997)
M Kosel et al.
Journal of chromatography. B, Biomedical sciences and applications, 719(1-2), 234-238 (1998-12-30)
A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma. Citalopram, demethylcitalopram and didemethylcitalopram, as well as the internal
Sherri L MacLeod et al.
Journal of chromatography. A, 1170(1-2), 23-33 (2007-10-05)
A reversed-phase enantioselective liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was developed to measure enantiomer fractions (EF) and concentrations of pharmaceuticals in wastewater. Enantiomer resolution of six beta-blockers (atenolol, metoprolol, nadolol, pindolol, propranolol, and sotalol) along with two selective serotonin re-uptake
Analysis of the enantiomers of citalopram and its demethylated metabolites using chiral liquid chromatography
Kosel, M., et al.
Journal of Chromatography. B, Biomedical Applications, 719 (1-2), 234-238 (1998)

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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What solvent to use for storage? Where can I download the user manual?

    1 answer
    1. Methanol is the recommended solvent for flushing and storage. The data sheet for this column is included with it upon shipment. A digital version can be obtained by contacting technical service.

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