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D8418

Dimethyl sulfoxide

Molecular Biology

Synonym(s):

Methyl Sulfoxide, Methylsulfinylmethane, Molecular Biology Grade DMSO, DMSO

เข้าสู่ระบบ เพื่อดูราคาสำหรับองค์กรและสัญญา

เลือกขนาด

เปลี่ยนการดู
ขนาด/SKUความพร้อมจำหน่ายราคา
50 mL

คาดว่าจะเริ่มจัดส่งในวันที่19 ตุลาคม 2569

เข้าสู่ระบบเพื่อดูราคา
100 mL

คาดว่าจะเริ่มจัดส่งในวันที่19 ตุลาคม 2569

เข้าสู่ระบบเพื่อดูราคา
250 mL

คาดว่าจะเริ่มจัดส่งในวันที่19 ตุลาคม 2569

เข้าสู่ระบบเพื่อดูราคา
500 mL

คาดว่าจะเริ่มจัดส่งในวันที่19 ตุลาคม 2569

เข้าสู่ระบบเพื่อดูราคา
1 L

คาดว่าจะเริ่มจัดส่งในวันที่19 ตุลาคม 2569

เข้าสู่ระบบเพื่อดูราคา

เกี่ยวกับสินค้านี้

สูตรเชิงเส้น:
(CH3)2SO
CASE番号:
น้ำหนักโมเลกุล:
78.13
UNSPSC Code:
12191502
NACRES:
NA.52
PubChem Substance ID:
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
Assay:
≥99.9%
Grade:
Molecular Biology
Technique(s):
DNA sequencing: suitable, PCR: suitable, transfection: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)


คาดว่าจะเริ่มจัดส่งในวันที่19 ตุลาคม 2569รายละเอียด


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grade

Molecular Biology

Quality Segment

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line

BioReagent

assay

≥99.9%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable, PCR: suitable, transfection: suitable

impurities

≤0.001 meq/g Titratable acid, ≤0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

absorption

passes test

suitability

suitable for molecular biology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

General description

Features and Benefits:

  • Suitable for Molecular Applications
  • Nuclease-Free DMSO
  • DNase-Free, RNase-Free, Protease-Free, Phosphatase-Free
  • Molecular Biology Grade DMSO

Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
This product is designated as Molecular Biology grade and is suitable for molecular biology applications. It has been analyzed for the presence of nucleases and proteases.

Application

DMSO (dimethyl sulfoxide) is a highly polar, aprotic organic solvent with many applications in organic chemistry and molecular biology. DMSO is routinely used in

  • polymerase chain reaction (PCR)
  • amplification of cDNA libraries
  • DNA sequencing
  • column-loading buffers for poly(A)+ RNA selection
  • buffers for the transformation of competent E. coli
  • transfection protocols.

Dimethyl sulfoxide has been used-

  • as an oligonucleotide solvent in array spotting[1]
  • as a solvent during miRNA microarray analysis and quantitative real-time PCR (qRT-PCR)
  • for cell lysis during MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay
  • for the preparation of the stock solution during MMP (mitochondrial membrane potential)cytofluorimetric assessment
  • in the storage of human and animal cell lines and bacteriophage λ as a cryoprotective agent.
To prepare a sterile filtered DMSO solution, it is recommended to use a PTFE or nylon membrane. Cellulose acetate membranes are not recommended.

Features and Benefits

This product is free from DNases, RNases, phosphatases and proteases.

Disclaimer

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

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สินค้าชิ้นนี้
PHR1309D917094563
Dimethyl sulfoxide Molecular Biology

Sigma-Aldrich

D8418

Dimethyl sulfoxide

Dimethyl Sulfoxide Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1309

Dimethyl Sulfoxide

Dimethyl sulfoxide PCR Reagent

Sigma-Aldrich

D9170

Dimethyl sulfoxide

Dimethyl sulfoxide analytical standard

Supelco

94563

Dimethyl sulfoxide

description

Molecular Biology

description

Pharmaceutical Secondary Standard; Certified Reference Material

description

PCR Reagent

description

analytical standard

technique(s)

transfection: suitable, DNA sequencing: suitable, PCR: suitable

technique(s)

gas chromatography (GC): suitable, HPLC: suitable

technique(s)

PCR: suitable

technique(s)

gas chromatography (GC): suitable, HPLC: suitable

assay

≥99.9%

assay

-

assay

≥99.5%

assay

≥99.95% (GC)

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

100

grade

Molecular Biology

grade

pharmaceutical secondary standard, certified reference material

grade

-

grade

analytical standard

solubility

H2O: miscible (completely)

solubility

-

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

form

liquid

form

-

form

liquid

form

-


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คลาสการจัดเก็บ

10 - Combustible liquids

จุดติดไฟ (°F)

188.6 °F - closed cup

จุดวาบไฟ (°C)

87 °C - closed cup

อุปกรณ์ป้องกันส่วนบุคคล (PPE)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



เลือกจากเวอร์ชันล่าสุด:

ใบรับรองผลการวิเคราะห์ (COA)

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Questions

1–10 of 19 Questions  
  1. Is this product anhydrous?

    1 answer
    1. This product is not anhydrous. The maximum allowable water content is < 0.1%. Please see the link below to review an anhydrous product option.
      276855
      https://www.sigmaaldrich.com/product/sial/276855

      Helpful?

  2. May I know the concentration (mg/ml) for DMSO (cat no. D8418)?

    1 answer
    1. This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.

      Helpful?

  3. Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?

    1 answer
    1. Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:

      -Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      -Moderately compatible: polystyrene, ECTFE/ETFE
      -Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf

      Helpful?

  4. Is this product sterile?

    1 answer
    1. This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
      https://www.sigmaaldrich.com/product/sigma/d2650
      https://www.sigmaaldrich.com/product/sigma/d2438

      Helpful?

  5. What can I use to solubilize Product D8418, Dimethyl sulfoxide?

    1 answer
    1. This product is miscible in water (1 mL DMSO + 1 mL H2O), yielding a clear, colorless solution. DMSO is a very hygroscopic liquid and should protected from exposure to moisture. DMSO is also miscible with ethanol, acetone, ether, benzene, and chloroform.

      Helpful?

  6. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  7. DMSO, Product D8418 Dimethyl sulfoxide, is used to freeze and store human and animal cell lines. Is there a procedure for it?

    1 answer
    1. The procedure to freeze cells is in the product information sheet (under Documents, above).

      Helpful?

  8. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  9. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  10. Is Dimethyl sulfoxide a sterile product?

    1 answer
    1. No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

      Helpful?

1–10 of 19 Questions  

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