IEVH85R
Immobilon® -E PVDF Membrane
1 roll, 8.5 cm × 10 m, 0.45 µm pore size, transfer membrane wets out in transfer buffer or water
Synonym(s):
Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane
Select a Size
About This Item
Skip To
Product Name
Immobilon®-E PVDF Membrane, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
material
PVDF membrane
plain filter
white filter
Quality Level
feature
easily wetted with water
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
filter L × W
8.5 cm × 10 m
pore size
0.45 μm pore size
compatibility
for use with Amido black
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Transillumination
detection method
chemiluminescent
colorimetric
radioactive
shipped in
ambient
1 of 4
This Item | IEVH07850 | IEVH00005 | IEVH09120 |
|---|---|---|---|
| material PVDF membrane, white filter, plain filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter | material PVDF membrane, plain filter, white filter |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size | pore size 0.45 μm pore size |
| compatibility for use with Amido black, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Transillumination, for use with Colloidal gold, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with India ink | compatibility for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination | compatibility for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination | compatibility for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination |
| detection method colorimetric, radioactive, chemiluminescent | detection method chemiluminescent, colorimetric, radioactive | detection method chemiluminescent, colorimetric, radioactive | detection method chemiluminescent, colorimetric, radioactive |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® |
General description
Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most Western blotting applications, especially proteins greater than 20 kDa
- Compatible with chemiluminescent and chromogenic detection
Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes.
Features & Benefits:
- High protein binding provides stronger signal
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be stripped and reprobed multiple times
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
討論影響凝膠電泳和 Western 印跡中觀察分子量與計算分子量的因素。
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Protocols
本頁顯示並討論三種剝離和重新印製 western 印迹膜的方案。
聚丙烯酰胺凝胶化学性质概述以及使用mPAGE® TurboMix Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
Related Content
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service