DUO82049
Duolink® In Situ Wash Buffers, Fluorescence
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About This Item
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material
packing (powdered buffer pouches)
product line
Duolink®
technique(s)
proximity ligation assay: suitable
suitability
suitable for fluorescence
storage temp.
20-25°C
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This Item | DUO82047 | DUO92010 | DUO92012 |
|---|---|---|---|
| technique(s) proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable | technique(s) immunofluorescence: suitable, proximity ligation assay: suitable | technique(s) proximity ligation assay: suitable |
| product line Duolink® | product line Duolink® | product line Duolink® | product line Duolink® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| storage temp. 20-25°C | storage temp. 20-25°C | storage temp. −20°C | storage temp. −20°C |
| suitability suitable for fluorescence | suitability suitable for brightfield | suitability suitable for brightfield, suitable for fluorescence | suitability suitable for brightfield |
| material packing (powdered buffer pouches) | material packing (powdered buffer pouches) | material - | material - |
Application
Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Duolink® In Situ fluorescence applications use two wash buffers. Wash Buffer A is used after the PLA Probe incubation step and Wash Buffer B is used after incubation with the amplification reagents. See datasheet for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Linkage
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Legal Information
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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Articles
包括建议和技巧、常见问题解答和基本故障排除的支持信息。
正确地准备、设置和执行 Duolink® PLA 实验方案需要考虑的因素。
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Things to consider for preparation, setup and execution of the Duolink® assay protocol
Protocols
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
Duolink® PLA Multicolor Detection Protocol
This page details the Duolink® In Situ Short Protocol for fluorescence detection
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View an automated protocol for Duolink® assays on the AAW™ automated assay workstation and see results comparing manual vs automated runs.
Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay
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