An amount of this product would need to be added to water in order to create the concentration of the desired buffer (e.g. 157.60 grams for one liter of a 1 M solution). The pH can then be adjusted as necessary.
648317
三盐酸盐，分子生物学级
Useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.
同義詞:
Trizma® 盐酸盐, TRIS 盐酸盐, 三羟基甲基氨基甲烷 盐酸盐, 三羟甲基氨基甲烷盐酸盐, 盐酸三甲胺
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線性公式:
NH2C(CH2OH)3 · HCl
CAS 編號:
分子量：:
157.60
NACRES:
NA.25
UNSPSC Code:
12161705
Beilstein/REAXYS Number:
3675235
MDL number:
暫時無法取得訂價和供貨情況
grade
Molecular Biology
Quality Segment
description
RTECS - TY2900000
assay
≥99% (titration)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic)
color
white
useful pH range
7.0-9.0
pKa (25 °C)
8.1
solubility
water: soluble
cation traces
heavy metals (as Pb): ≤0.0002%
absorption
≤0.05 at 260 nm, ≤0.05 at 280 nm
foreign activity
DNases, proteases, RNases, none detected
shipped in
ambient
storage temp.
15-25°C
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
General description
在7.0-9.0的pH范围内有效。25°C时PKa为8.1。
广泛使用的生物缓冲液（吸光度：≤260 nm和280 nm处为0.05）。在7.0-9.0的pH范围内有效。25°C时PKa为8.1。
Other Notes
所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液，温度每降低 1°C，pH 就会增加约 0.03 单位，每稀释 10 倍，pH 会降低 0.03-0.05 单位。对于一些精准的应用，请使用经过仔细校准的pH计和玻璃/甘汞组合电极。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性：标准处理（A）
存儲類別/等級
11 - Combustible Solids
到底發生了什麼事？
WGK 1
閃點 (F)
Not applicable
閃點（°C）
Not applicable
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How can i prepare the buffer HCL solution?
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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