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Merck

CC080

Human Vitronectin

from human plasma, liquid, 1 mg/mL, suitable for cell culture

同義詞:

S-protein

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分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

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產品名稱

Vitronectin, Human Purified Protein,

生物源

human

品質等級

化驗

≥90% (SDS-PAGE)

形狀

liquid

比活性

1 μg/mL (cell attachment assays)

製造商/商標名

Chemicon®

濃度

1 mg/mL

技術

cell culture | mammalian: suitable

輸入

sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... VTN(7448)

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本產品
5051CC130FC010
biological source

human

biological source

human plasma

biological source

Escherichia coli, human

biological source

human

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

concentration

1 mg/mL

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

1 mg/mL

assay

≥90% (SDS-PAGE)

assay

>95%

assay

≥92% (SDS-PAGE)

assay

~95% (SDS-PAGE)

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

liquid

form

solution

form

liquid

form

(liquid: 1MG, 5MG, 10MG pack size
lyophilized: 100 MG pack size)

一般說明

PROPERTIES:

Binds to heparin. Active in cell attachment of MG63 cells.
Product Source: Human plasma; tested negative for antibodies to HBsAg and HIV.
Vitronectin (S-protein) is a multi-functional plasma and extracellular matrix protein with defined activity in cell adhesion, thrombosis, complement activation, fibrinolysis, inflammation, and platelet adhesion. Vitronectin binds to other macromolecules and participates in regulatory circuits to control these processes. Some target macromolecules that interact with vitronectin are heparin, PAI-1, proteases such as thrombin and urokinase-type plasminogen activator (uPA), serine protease inhibitor-protease complexes, the uPA receptor, and a sub-class of integrin receptors on the surface of cells. Vitronectin is also applied as a thin coating on tissue-culture surfaces, which is useful to promote cell attachment, spreading, proliferation, and differentiation of many normal and neoplastic cells, and to study cell migration.

應用

Suitable for use in ELISA, immunization, cell attachment assays and electrophoresis.

外觀

Liquid in 50 mM sodium phosphate, 100 mM NaCl, pH 7.4, containing no preservatives.

準備報告

Stable 12 months at -70°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

分析報告

Two bands in SDS-PAGE at 65 kDa and 75 kDa.

其他說明

Replaces: 08-126

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Lynn Yap et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 106(5), 1887-1896 (2017-09-25)
Functionalizing medical devices with polypeptides to enhance their performance has become important for improved clinical success. The extracellular matrix (ECM) adhesion protein vitronectin (VN) is an effective coating, although the chemistry used to attach VN often reduces its bioactivity. In
Carolina Epifano et al.
EMBO reports, 15(5), 592-600 (2014-03-19)
The adherens junction protein p120-catenin is implicated in the regulation of cadherin stability, cell migration and inflammatory responses in mammalian epithelial tissues. How these events are coordinated to promote wound repair is not understood. We show that p120 catenin regulates
Jian Li et al.
Biointerphases, 5(3), FA132-FA142 (2010-12-22)
The standard method for culturing human embryonic stem cells (hESC) uses supporting feeder layers of cells or an undefined substrate, Matrigel(™), which is a basement membrane extracted from murine sarcoma. For stem cell therapeutic applications, a superior alternative would be
Morten Aagaard Nielsen et al.
Frontiers in immunology, 13, 915890-915890 (2022-07-12)
4-1BB is a T cell costimulatory receptor and a member of the tumor necrosis factor receptor superfamily. Here, we show that Galectin-3 (Gal-3) decreases the cellular response to its ligand (4-1BBL). Gal-3 binds to both soluble 4-1BB (s4-1BB) and membrane-bound
Andrew J Putnam et al.
Cell communication and signaling : CCS, 7, 10-10 (2009-04-30)
Integrins, cell-surface receptors that mediate adhesive interactions between cells and the extracellular matrix (ECM), play an important role in cancer progression. Expression of the vitronectin receptor alphavbeta3 integrin correlates with increased invasive and metastatic capacity of malignant melanomas, yet it

條款

本頁涵蓋針對 Millicell®-CM 插片上四種類型 ECM(1 型膠原蛋白、纖維連結蛋白、層粘結蛋白和 Matrigel)所開發的 ECM 塗佈方案。

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

相關內容

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

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