HDIAB-34K-PMX5

MILLIPLEX^® Human Diabetes Panel, Premixed 5 Plex Assay

The Human Diabetes, Premixed 5 Plex Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 protein biomarkers in human serum, plasma and cell culture samples.

• 分類程式碼代碼: 12161503
• eCl@ss: 32161000

屬性

• 物種活性: human
• 製造商／商標名: Milliplex^®
• assay range: accuracy: 86%; (GLP-1 (Active)); accuracy: 91%; (Insulin); accuracy: 92%; (C-Peptide); accuracy: 94%; (Leptin); sensitivity: 15.8 pg/mL; (MinDC+2SD; Glucagon); sensitivity: 16.4 pg/mL; (MinDC+2SD; C-Peptide); sensitivity: 2.1 pg/mL; (MinDC+2SD; GLP-1 (Active)); sensitivity: 32.7 pg/mL; (MinDC+2SD; Insulin); sensitivity: 65.9 pg/mL; (MinDC+2SD; Leptin); standard curve range: 13.7-10,000 pg/mL; (Glucagon); standard curve range: 137-100,000 pg/mL; (Insulin); standard curve range: 137-100,000 pg/mL; (Leptin); standard curve range: 2.7-2,000 pg/mL; (GLP-1 (active)); standard curve range: 27.4-20,000 pg/mL; (C-Peptide); inter-assay cv: <15%; intra-assay cv: <10%; (C-Peptide); inter-assay cv: <15%; intra-assay cv: <10%; (Glucagon); inter-assay cv: <15%; intra-assay cv: <10%; (Insulin); inter-assay cv: <15%; intra-assay cv: <10%; (Leptin); inter-assay cv: <20%; intra-assay cv: <10%; (GLP-1 (Active))
• 技術: multiplexing: suitable
• 相容性: configured for Premixed
• 檢測方法: fluorometric (Luminex xMAP)
• 運輸包裝: wet ice

描述

一般說明

Type I Diabetes is an early onset autoimmune disease that destroys insulin producing pancreatic β-cells. Type II Diabetes is later onset metabolic disorder caused by obesity, poor diet and a sedentary lifestyle. With insufficient levels of insulin, or resistance to insulin, elevated glucose can lead to heart disease, stroke, kidney failure and dementia.

MILLIPLEX^® Human Diabetes Premixed Bead Panel is a 5-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma, or culture supernatant samples: C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin.

This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

應用

• Analytes: C-Peptide, Glucagon-like Peptide-1 (GLP-1) (Active), Glucagon, Insulin, Leptin
• Recommended Sample Type: Human serum, plasma, tissue/cell culture supernatants and lysates
• Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require a dilution with an appropriate control medium.
• NOTE: Protease inhibitors must be added to blood upon collection, DPPIV inhibitor for GLP-1 and Aprotinin for Glucagon. See protocol for details.
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Metabolism
• Research Subcategory: Obesity, Diabetes, Diabetes - Type 1, Diabetes - Type 2

生化／生理作用

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

包裝

Everything you need in a single kit.

準備報告

Recommended storage for kit components is 2 - 8°C.

其他說明

Please contact Technical Service for linearity of dilution.

法律資訊

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS06,GHS09
• 訊號詞: Danger
• 危險聲明: H302 + H332,H311,H317,H319,H411
• 防範說明: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• 危險分類: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects