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IP02

蛋白A琼脂糖混悬剂

Name: 蛋白A琼脂糖混悬剂
Brand: MM

Protein A Agarose Suspension designed for immunoprecipitation applications

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NACRES:

NA.56

UNSPSC Code:

41116133

eCl@ss:

39020601

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屬性

form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

technique(s)

antibody protein purification: suitable

shipped in

wet ice

storage temp.

2-8°C

描述

General description

与琼脂糖结合的蛋白A。

专为免疫沉淀应用而设计。本品用BSA封闭，减少非特异性结合，不能用于纯化；对小鼠IgG_2a和IgG_2b及兔IgG效果最好。

Physical form

在PBS中。

Other Notes

琼脂糖溶液为即用型，可用于免疫沉淀。使用15µ l混悬剂/µg一抗。推荐将蛋白A-琼脂糖用于使用兔多克隆抗体或未修饰的小鼠IgG_2a或IgG_2b单克隆抗体的免疫沉淀测定。小鼠IgG₁或IgG₃和全部大鼠抗体与蛋白A的结合较弱。作为替代方案，使用蛋白G PLUS，蛋白G PLUS/蛋白A或兔第二步抗体将这些抗体与蛋白A桥接。预清除将使由非特异性沉淀产生额外的条带最小化。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与BSA或样品中存在的其他高浓度蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性：标准处理（A）

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本產品	IP04	IP05	IP10
Millipore IP02 蛋白A琼脂糖混悬剂	Millipore IP04 蛋白质G Plus-琼脂糖悬浮液	Millipore IP05 蛋白质G Plus /蛋白质A琼脂糖悬浮液	Millipore IP10 蛋白G Plus/蛋白A-琼脂糖
description Protein A Agarose Suspension designed for immunoprecipitation applications	description Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.	description Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.	description A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.
technique(s) antibody protein purification: suitable	technique(s) antibody protein purification: suitable, immunoprecipitation (IP): suitable	technique(s) antibody protein purification: suitable	technique(s) antibody protein purification: suitable
form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative
storage condition do not freeze	storage condition do not freeze	storage condition do not freeze	storage condition do not freeze

存儲類別/等級

11 - Combustible Solids

WGK

WGK 1

閃點 (F)

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

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