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IP02

蛋白A琼脂糖混悬剂

Name: 蛋白A琼脂糖混悬剂
Brand: MM

Protein A Agarose Suspension designed for immunoprecipitation applications

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NACRES:

NA.56

UNSPSC Code:

41116133

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屬性

form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

technique(s)

antibody protein purification: suitable

shipped in

wet ice

storage temp.

2-8°C

描述

General description

与琼脂糖结合的蛋白A。

专为免疫沉淀应用而设计。本品用BSA封闭，减少非特异性结合，不能用于纯化；对小鼠IgG_2a和IgG_2b及兔IgG效果最好。

Physical form

在PBS中。

Other Notes

琼脂糖溶液为即用型，可用于免疫沉淀。使用15µ l混悬剂/µg一抗。推荐将蛋白A-琼脂糖用于使用兔多克隆抗体或未修饰的小鼠IgG_2a或IgG_2b单克隆抗体的免疫沉淀测定。小鼠IgG₁或IgG₃和全部大鼠抗体与蛋白A的结合较弱。作为替代方案，使用蛋白G PLUS，蛋白G PLUS/蛋白A或兔第二步抗体将这些抗体与蛋白A桥接。预清除将使由非特异性沉淀产生额外的条带最小化。要进行预清除，将与免疫沉淀抗体相同物种的 20 µl 琼脂糖结合物和 1 µg 正常 IgG 加入样品中。当使用免疫印迹进行检测时，某些二抗可以与BSA或样品中存在的其他高浓度蛋白质发生非特异性反应。这可以通过降低二抗的浓度来消除。

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

毒性：标准处理（A）

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本產品	IP04	IP05	IP10
Millipore IP02 蛋白A琼脂糖混悬剂	Millipore IP04 蛋白质G Plus-琼脂糖悬浮液	Millipore IP05 蛋白质G Plus /蛋白质A琼脂糖悬浮液	Millipore IP10 蛋白G Plus/蛋白A-琼脂糖
description Protein A Agarose Suspension designed for immunoprecipitation applications	description Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.	description Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.	description A mixture of Protein G PLUS and Protein A covalently conjugated to agarose. Useful for purification of IgG from biological fluids.
form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®
storage condition do not freeze	storage condition do not freeze	storage condition do not freeze	storage condition do not freeze
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

存儲類別/等級

11 - Combustible Solids

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WGK 1

閃點 (F)

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