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Merck

4381663

iTRAQ® Reagent - 8PLEX Multiplex Kit

同義詞:

iTRAQ 8-Plex

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NACRES:
NA.24
UNSPSC Code:
12161503

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本產品
437477143816624369561
Quality Level

100

Quality Level

200

Quality Level

-

Quality Level

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

含有iTRAQ®试剂113、114、115、116、117、118、119及121,以及足够进行5次8重检测的材料。多重缓冲液试剂盒(货号4381664)需另行订购。

货号4381663产品所含试剂相当于Sciex iChemistry®货号4390812的产品。
相对和绝对定量同位素标记(isobaric tags for relative and absolute quantitation)iTRAQ®试剂是首个多重、胺特异性、稳定同位素试剂组合,可同时标记8种不同生物学样本中的所有多肽以进行鉴定及相对和绝对定量,并且保留重要的翻译后修饰(PTM)信息。iTRAQ 8重试剂盒有更经济的大包装可供选择。

Application

iTRAQ® Reagent - 8PLEX Multiplex Kit 8标蛋白质多重检测试剂盒已用于:
  • 衍生化和标记碘代乙酰胺探针(IPM)标记的HeLa蛋白组的胰酶消化物[1]
  • 相对和绝对定量同位素标记(iTRAQ)多肽[2]
  • 作为蛋白组分析的同量异位素(isobar)标记,用于不同消化样本蛋白的比较定量[3]

Features and Benefits

  • 同时对多个样本中的蛋白质进行鉴定和定量
  • 标记所有肽段以扩大蛋白和蛋白组覆盖率,包括翻译后修饰(PTM)分子
  • 标记每个蛋白的多个肽,提高MS/MS质谱鉴定和定量的可靠性
  • 提高结果通量和可靠性,便于进行蛋白质生物标志物研究
  • 流程简化,无需进行样品分离以降低样本复杂性,比如蛋白质亲和沉降技术(affinity pull-down)
  • 一次实验最多可同时检测8种不同的生物样本
  • ProteinPilot软件全面支持所有的SCIEX蛋白组学LC/MS/MS平台

Analysis Note

查看iTRAQ试剂 8-plex Kit,请访问该实验方案链接

查看iTRAQ试剂化学快速参考卡,请访问该化学快速参考卡链接

Legal Information

iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.

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Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2

存儲類別/等級

3 - Flammable liquids

flash_point_f

35.6 °F - closed cup

flash_point_c

2.0 °C - closed cup

wgk

WGK 3


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He Wang et al.
Archives of oral biology, 89, 9-19 (2018-02-07)
This study aimed to compare the proteomic profiling of human dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) under in vitro osteogenic induction, which imitates the microenvironment during osteo-/odontogenesis of DPSCs and PDLSCs. The proteomic profiles of
Hongzhi Liu et al.
Oncology letters, 16(1), 335-345 (2018-06-22)
Metastasis and recurrence following surgery are major reasons for the high mortality rate and poor prognosis associated with hepatocellular carcinoma (HCC). Cancer stem cells (CSCs) are thought to be able to cause cancer, and to be the primary cause of
Shaoli Liu et al.
Journal of microbiology and biotechnology, 30(2), 187-195 (2019-11-23)
To understand the molecular mechanism involved in the survivability of cold-tolerant lactic acid bacteria was of great significance in food processing, since these bacteria play a key role in a variety of low-temperature fermented foods. In this study, the cold-stress
Jesús Alejandro Zamora-Briseño et al.
Frontiers in microbiology, 11, 1084-1084 (2020-06-18)
The Caribbean spiny lobster Panulirus argus (Latreille, 1084) sustains economically valuable fisheries throughout the wider Caribbean region. This species is currently affected by the pathogenic virus Panulirus argus Virus 1 (PaV1) that causes a systemic and chronic-degenerative infection in juvenile
Juan Jin et al.
Biomarkers in medicine, 14(10), 839-854 (2020-08-29)
Background: Proteome studies for multiple renal diseases is bare. Methodology & results: Using isobaric tags for relative and absolute quantitation labeling, many differentially expressed proteins (DEPs) were identified in acute kidney injury (AKI), AKI + chronic kidney disease (CKD), diabetic CKD and

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