The difference between the Type I and Type II Asialofetuin products does not represent any kind of biochemical or functional difference. These indicate different methods of production and purification. The Type II option, product A1908, utilizes neuraminadase in an enzymatic cleavage step. The Type I, product A4781, utilizes a proprietary modification of that described in Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).
biological source
bovine
Quality Segment
type
Type II (Sigma designation)
form
powder
technique(s)
activity assay: suitable, inhibition assay: suitable
impurities
salt, essentially free, ≤1.0% N-acetylneuraminic acid
solubility
H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow
storage temp.
2-8°C
Application
Asialofetuin from fetal calf serum is suitable for use:
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.
Biochem/physiol Actions
Feutins are glycoproteins that belong to the cystatin superfamily. They are characterized by the presence of two tandemly arranged cystatin-like domains and an unrelated domain. It binds to transforming growth factor (TGF)-β/BMP (bone morphogenic protein) cytokines.
Asialofetuin (AF) binds to asialoglycoprotein receptor (ASGP-R) and is employed as a ligand for drug delivery to the hepatocytes. It aids serum-resistant lipoplexes for efficient gene deliver into the HepG2 (human hepatoblastoma) cells.
Preparation Note
Enzymatic cleavage using neuraminidase
1 of 1
本產品 | |||
|---|---|---|---|
| description Type II (Sigma designation) | description lyophilized powder | description lyophilized powder, suitable for cell culture | description lyophilized powder, BioReagent, suitable for cell culture |
| biological source bovine | biological source bovine serum (fetal) | biological source bovine serum (fetal) | biological source - |
| technique(s) activity assay: suitable, inhibition assay: suitable | technique(s) HPLC: suitable, MALDI-MS: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| impurities ≤1.0% N-acetylneuraminic acid, salt, essentially free | impurities ~0.2% free N-acetylneuraminic acid | impurities ≤0.3% free N-acetylneuraminic acid | impurities ≤0.3% free N-acetylneuraminic acid |
| solubility H2O: soluble 1.0 mg/mL, clear to hazy, colorless to very faintly yellow | solubility - | solubility H2O: 1 mg/mL, colorless to faint yellow or tan | solubility H2O: 1 mg/mL |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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存儲類別/等級
11 - Combustible Solids
到底發生了什麼事？
WGK 3
閃點 (F)
Not applicable
閃點（°C）
Not applicable
PPE（個人防護裝備）
Eyeshields, Gloves, type N95 (US)
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What is the difference in Type 1 versus Type II asialofetuin?
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