C1907

钙调磷酸酶 来源于牛大脑

lyophilized powder, ≥2,500 units/mg protein

• 同義詞: PP2B, 磷蛋白磷酸水解酶, 蛋白磷酸酶 2B, 调节因子结合蛋白, 钙/钙调素活化的蛋白磷酸酶, 钙调素结合蛋白
• CAS 編號: 90371-51-0
• UNSPSC Code: 51111800
• NACRES: NA.32
• MDL number: MFCD00130697
• Specific activity: ≥2,500 units/mg protein
• Biological source: bovine

屬性

• biological source: bovine
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥2,500 units/mg protein
• mol wt: dimer ~77 kDa, subunit mol wt 19-58 kDa
• composition: Protein, 0.3-1.7% Lowry
• solubility: H₂O: soluble
• UniProt accession no.: P48452
• storage temp.: −20°C
• Gene Information: cow ... PPP3CA(286852)

描述

General description

Calcineurin (CaN) comprises CaN A and CaN B subunits. It exists as a heterodimer with a calmodulin-binding domain, catalytic site, a CaN B binding domain, and an autoinhibitory domain.[1]

Application

Calcineurin from bovine brain has been used:

• as a positive control in western blot analysis of oocytes and cumulus cells proteome[2]
• as a positive control in calmodulin (CaM)-agarose binding assay
• to test its phosphatase activity in the presence of okadaic acid[3]

Biochem/physiol Actions

大脑中发现的主要钙调素结合蛋白。与 T 细胞活化和阿尔茨海默病中 τ 蛋白的过磷酸化有关的关键酶。

钙调磷酸酶是一种环孢菌素敏感、钙调节的丝氨酸-苏氨酸蛋白质磷酸酶，具有较广的底物特异性。它是大脑中发现的主要钙调素结合蛋白。最早鉴定为一种磷酸二酯酶 3′:5′ 环核苷酸 (PDE) 的钙调素活化抑制剂，钙调磷酸酶对腺苷酸环化酶具有类似的作用。是与 T 细胞活化相关的关键酶。也与阿尔茨海默病中 τ 蛋白的过磷酸化有关，并且已显示出可阻碍分化型 PC12 细胞中 τ 蛋白进行钙蛋白酶介导的蛋白质水解的特性。

Calcineurin (CaN) activity is stimulated by nickel (Nⁱ²⁺) and manganese (Mn²⁺) ions.[1] It participates in the coupling of Ca²⁺ signals.[1] CaN may regulate oocyte growth and meiotic maturation in porcine.[2] It also participates in gene regulation, cell survival, and death.[3]

Physical form

冻干粉，含 0.5% EGTA，缓冲盐及稳定剂。

Analysis Note

Sigma 在含 65mM KCl、8mM MgSO₄ 以及 0.3% 白蛋白且 pH 为 7.5 的 80mM Tris 中测试了其活性。

Other Notes

当与两单位活化剂 (P 2277) 以及 0.1mM Ca²⁺ 置于酶偶联系统中分析时，在 pH 7.5 和 30°C 下，一单位本品可以对活化的磷酸二酯酶 3′:5′-环核苷酸 (P 9529) 造成 50% 的抑制。

安全資訊

• 存儲類別/等級: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves