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P1415

预杂交溶液

Name: 预杂交溶液
Brand: SIGMA

for Northern and Southern blotting, liquid (2x concentrate)

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關於此項目

NACRES:

NA.25

UNSPSC Code:

12161700

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屬性

Quality Segment

100

form

liquid (2x concentrate)

technique(s)

nucleic acid detection: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

-10 to -25°C

描述

General description

Sigma的预杂交液是一种2倍浓缩的即用型杂交缓冲液，适用于Northern和Southern印迹方案。

Application

适合用作Northern和Southern印迹方案的预杂交缓冲液。

Preparation Note

Sigma的2倍预杂交液含200 μg/mL DNA，溶剂为10倍SSC和2倍Denhardt溶液：

200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg Ficoll
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5M NaCl

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本產品	H7140	D2532	D9905
Sigma-Aldrich P1415 预杂交溶液	Sigma-Aldrich H7140 杂交溶液	Sigma-Aldrich D2532 Denhardt溶液 50x	Sigma-Aldrich D9905 Denhardt′s溶液
description for Northern and Southern blotting, liquid (2x concentrate)	description for Northern and Southern blotting, liquid (2x concentrate)	description for Northern and Southern blotting, solution	description for Northern and Southern blotting, lyophilized powder
technique(s) nucleic acid detection: suitable	technique(s) nucleic acid detection: suitable	technique(s) Southern blotting: suitable, nucleic acid detection: suitable	technique(s) Southern blotting: suitable, nucleic acid detection: suitable
Quality Level 100	Quality Level 100	Quality Level 300	Quality Level 100
form liquid (2x concentrate)	form liquid (2x concentrate)	form solution	form lyophilized powder
storage temp. -10 to -25°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected	foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected	foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected	foreign activity DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

存儲類別/等級

10 - Combustible liquids

WGK

WGK 2

閃點 (F)

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number

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