I bought this kit as replacement of NA0410-1Kt which I was waiting for 1 year and still wasn't able to get due to delay. Plex15 is significantly more expensive, uncomfortable to work with and yields less plasmid DNA. I regret I had to waste my time and money on it.
usage
sufficient for 15 purifications
Quality Segment
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Sustainability features
technique(s)
DNA purification: suitable
test parameters
: <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture
storage temp.
15-25°C
General description
GenElute无内毒素质粒制备试剂盒可以简单、快速、低成本的纯化质粒DNA（内毒素水平≤0.1 EU/μg），所得产物可用于高效转染。 内毒素（也称为脂多糖或LPS）常常会与质粒DNA共纯化，这会显著降低内毒素敏感细胞系的转染效率。
取过夜培养物离心收集重组大肠杆菌，采用改良的SDS碱裂解法处理得到澄清的裂解液。通过简单的萃取相分离步骤去除内毒素。在高盐环境下，质粒DNA吸附到硅胶膜上接受进一步的纯化。简单的离心洗涤后，用无内毒素的水洗脱吸附的质粒DNA。25-200 mL培养物可以制备出多达1.2 mg不含内毒素的质粒DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接用于转染、限制性酶切、克隆、测序和PCR扩增等下游应用。
取过夜培养物离心收集重组大肠杆菌，采用改良的SDS碱裂解法处理得到澄清的裂解液。通过简单的萃取相分离步骤去除内毒素。在高盐环境下，质粒DNA吸附到硅胶膜上接受进一步的纯化。简单的离心洗涤后，用无内毒素的水洗脱吸附的质粒DNA。25-200 mL培养物可以制备出多达1.2 mg不含内毒素的质粒DNA。
回收的质粒DNA主要以超螺旋形式存在。此类DNA可直接用于转染、限制性酶切、克隆、测序和PCR扩增等下游应用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。相比使用苯酚和氯仿的标准DNA提取方法，该产品是一种更安全的化学产品。
Application
该试剂盒已用于纯化质粒脱氧核糖核酸，用于高效转染。
Features and Benefits
- 转染效率高，产量高
- 纯化至 250μg 无内毒素质粒 DNA（≤0.1 EU/μg DNA）
- 快速简单，允许在 2 小时内完成 12 次制备
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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本產品 | |||
|---|---|---|---|
| description sufficient for 15 purifications | description sufficient for 15 purifications | description sufficient for 10 preparations | description sufficient for 25 preparations |
| usage sufficient for 15 purifications | usage sufficient for 15 purifications | usage sufficient for 10 preparations | usage sufficient for 25 preparations |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable |
| greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| greener alternative category , Aligned | greener alternative category | greener alternative category | greener alternative category |
| test parameters : <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture | test parameters sample volume: 25-200 mL bacterial culture, : ~45 min kit run time | test parameters sample volume: 150 mL bacterial culture | test parameters sample volume: 150 mL bacterial culture |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
套裝中的組件也可單獨購買
產品號碼
描述
SDS & 訂價
警示詞
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶向器官
Central nervous system
閃點 (F)
Not applicable
閃點（°C）
Not applicable
到底發生了什麼事？
WGK 3
存儲類別/等級
10 - Combustible liquids