1.14582
mEC-broth with Novobiocin
for microbiology
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Quality Level
form
medium granules (dehydrated (DCM))
manufacturer/tradename
GranuCult®
technique(s)
microbiological culture: suitable
pH
6.7-7.1 (25 °C, 36.7 g/L in H2O, after autoclaving)
solubility
36.7 g/L
application(s)
food and beverages
microbiology
pathogen testing
storage temp.
15-25°C
suitability
Escherichia coli
Categorías relacionadas
1 of 4
Este artículo | 1.05878 | 1.09205 | SBR00025 |
|---|---|---|---|
| suitability Escherichia coli | suitability selective for Salmonella spp. | suitability Escherichia coli (O157:H7 ), selective for Escherichia coli (EHEC), urea-degrading bacteria | suitability - |
| technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable | technique(s) - |
| form medium granules (dehydrated (DCM)) | form granular | form granular | form liquid |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. −20°C |
Analysis Note
Appearance (color): yellowish to yellowish-braun
pH-value (25 °C): 6.7 - 7.1
Inoculum on reference medium (Escherichia coli ATCC 35150 (O157)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 100
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 10000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 10000
Inoculum on reference medium (Proteus hauseri ATCC 13315): ≥ 10000
Growth (Escherichia coli ATCC 35150 (O157)): >10 transparent colonies with a pale yellowish-brown appearance on SMAC agar
Growth (Escherichia coli ATCC 11775 (WDCM 00090)): >10 pink colonies on SMAC Agar
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition on TSA
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition on TSA
Mixed inoculum of E.coli, P.hauseri and E.faecalis
Additionaly monoculture of E.faecalis
Confirmation on SMAC agar resp. Tryptic soy agar
Incubation:
mEC broth: 18 - 24 hours at 41,5 ± 1 °C aerobic
Confirmation on SMAC agar resp. Tryptic Soy Agar: 18 - 24 hours at 35 ± 1 °C aerobic
Other Notes
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
Clase de almacenamiento
11 - Combustible Solids
wgk_germany
WGK 3
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