Keep using our User Guide for guidance. A 3 kda membrane is extremely small and every 3 minutes counts in having water pass through the membrane. One would want to continue spinning at that speed or shortly below. In time, the initial volume does decrease. One cannot concentrate water. A purpose of using water initially is simply to wet the membrane before introducing a real sample in the hopes of decreasing adherence of the membrane to the substance to be concentrated. One can simply spin at 500 x g for a few moments then discard, then introduce the sample.
UFC5003
Filtro de ultracentrífuga Amicon®, MWCO 3 kDa
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 3 kDa
Sinónimos:
Concentrador para centrífuga, MWCO de 3 kDa, volumen de muestra de 0,5 ml
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24 PKG
$186.00
96 PKG
$591.00
500 PKG
$2,390.00
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UNSPSC Code:
41104916
eCl@ss:
36100101
NACRES:
NB.24
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Servicio técnico
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Permítanos ayudarlematerial
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
sterility
non-sterile
product line
Amicon®
packaging
pkg of 24 pkg
pkg of 500 pkg
pkg of 8 pkg
pkg of 96 pkg
manufacturer/tradename
Amicon® Ultra
sustainability
Greener Alternative Product
parameter
0.5 mL sample volume
technique(s)
protein extraction: suitable
protein purification: suitable
L
4.99 cm (1.96 in.)
diam.
10.8 mm
filtration area
1 cm2
volume
0.5 mL
working volume
≤500 μL
0.5 mL
pore size
3 kDa MWCO
greener alternative category
shipped in
ambient
1 of 4
Este artículo | UFC200324 | UFC9003 | UFC8003 |
|---|---|---|---|
| pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO | pore size 3 kDa MWCO |
| material polypropylene cap, regenerated cellulose membrane, polypropylene tube, styrene-butadiene housing | material polypropylene tube (for collection), regenerated cellulose membrane, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing | material polyethylene cap, polyethylene liner, polypropylene tube, regenerated cellulose membrane, regenerated cellulose support, styrene-butadiene housing |
| parameter 0.5 mL sample volume | parameter 2 mL sample volume | parameter 15 mL sample volume | parameter 4 mL sample volume |
| manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra | manufacturer/tradename Amicon® Ultra |
| sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| product line Amicon® | product line Amicon® | product line Amicon® | product line Amicon® |
General description
Las unidades de filtración mediante centrífuga Amicon® Ultra-0,5 proporcionan una ultrafiltración rápida, con la capacidad de altos factores de concentración y una fácil recuperación de los concentrados de las matrices de muestras diluidas y complejas. Por ejemplo, el citocromo C (12,4 kDa) a una concentración inicial de 0,25 mg/ml es retenido normalmente al >95 %.
El diseño vertical y la superficie de membrana disponible proporcionan un procesamiento rápido de la muestra, una elevada recuperación de la muestra (normalmente superior al 90 % de la disolución inicial diluida) y la capacidad de concentración de 30 veces. La polarización del soluto y el consiguiente ensuciamiento de la membrana se reducen al mínimo gracias al diseño vertical, y un bloqueo físico del filtro evita la centrifugación hasta la sequedad y la posible pérdida de muestra. La recuperación eficiente de la muestra concentrada (especies retenidas) se consigue mediante una cómoda etapa de espín inverso después de recoger el filtrado.
Las unidades Amicon® Ultra-0,5 se suministran sin esterilizar y son para un solo uso. Con su diseño de membrana vertical y espín inverso, los filtros Amicon® Ultra de 0,5 ml ofrecen un gran rendimiento y menores tiempos de centrifugado.
El diseño vertical y la superficie de membrana disponible proporcionan un procesamiento rápido de la muestra, una elevada recuperación de la muestra (normalmente superior al 90 % de la disolución inicial diluida) y la capacidad de concentración de 30 veces. La polarización del soluto y el consiguiente ensuciamiento de la membrana se reducen al mínimo gracias al diseño vertical, y un bloqueo físico del filtro evita la centrifugación hasta la sequedad y la posible pérdida de muestra. La recuperación eficiente de la muestra concentrada (especies retenidas) se consigue mediante una cómoda etapa de espín inverso después de recoger el filtrado.
Las unidades Amicon® Ultra-0,5 se suministran sin esterilizar y son para un solo uso. Con su diseño de membrana vertical y espín inverso, los filtros Amicon® Ultra de 0,5 ml ofrecen un gran rendimiento y menores tiempos de centrifugado.
Nos hemos comprometido a ofrecerle productos alternativos más ecológicos, que han sido rediseñados para permitir soluciones de investigación más sostenibles. El dispositivo de filtración interior está compuesto por material biocircular asignado mediante el enfoque de equilibrio de masas ISCC. Pulse aquí para más información.
Application
- Ultrafiltración con membrana Ultracel® de corte de peso molecular de 3 000 dalton
- Concentración de muestras biológicas que contienen antígenos, anticuerpos, enzimas, ácidos nucleicos o microorganismos
- Purificación de los componentes macromoleculares que se encuentran en extractos de cultivo tisular o lisados celulares, eliminación de cebadores, conectores o etiquetas macromoleculares de una mezcla de reacción y eliminación de las proteínas antes de la HPLC
- Desalación, cambio de la disolución tampón y diálisis de proteínas
Features and Benefits
- La membrana de celulosa regenerada Ultracel® con un NMWCO de 3 kDa proporciona una recuperación del retenido superior al 90 %
- Concentra desde 500 μl hasta 15 μl.
- Factor de concentración 25x a 30x
- Elevada recuperación de la muestra (superior al 90 % de la disolución diluida inicial)
- Tiempo de procesamiento rápido: 10 a 30 minutos
- La capacidad de espín inverso proporciona recuperaciones uniformes
Physical form
Código de colores: Transparente
Legal Information
Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Filtration methods and membrane filter selection in nanoparticle purification and production.
Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
Protocolos
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Contenido relacionado
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I centrifuged 200 µL of water (test) for 30min at 14,000g using a 45° fixed-angle rotor. 145 µL passed through, leaving 55 µL on top. How can I reduce this residual volume to 15 µL stated in the product's features? Increasing speed and time didn't help!
1 answer-
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I would like to use this device to remove protein from human serum. Afterwards I would need to use the filtered serum in cell culture conditions. Is there also a sterile version of this product available? Or can this product safely be autoclaved?
1 answer-
Amicon Ultra devices are used to concentrate substances in liquids. For example, if a protein of interest is 68 kDa, a device with a 30 kDa molecular weight cutoff would be used to concentrate it. The 68 kDa protein would remain on top of the filter and not pass through, while smaller substances might be present in both the filtrate and the retentate. These devices concentrate rather than purify. A sterile version of this product is not available, but a method to sanitize involves adding 70% isopropyl alcohol, spinning for a minute, and then discarding. This product cannot be autoclaved.
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What is the margin of error for the MWCO? I read somewhere the RECOMMENDATION is to use a MWCO 2-3x smaller than your target. But I was wondering what’s the standard deviation from 3 kDa?
1 answer-
For most membranes, the nominal molecular weight limit, also referred to as the molecular weight cut-off, is determined experimentally under a standard set of operating conditions. These analyses typically employ purified globular proteins to serve as markers or indicators of the retention characteristics of an ultrafiltration membrane. An ultrafiltration membrane with a stated nominal molecular weight limit should retain (reject) at least 90% of a globular solute of that molecular weight in daltons. However, for a wider safety margin, the selected cut-off should be well below the molecular weight of the solute to be retained. It is recommended to use a cut-off 2-3 times smaller than the molecular weight of the protein to be concentrated.
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Is this product compatible with organic solvents, such as DMSO or DMF?
1 answer-
The Amicon Ultra products are suitable for use with samples containing up to 10% DMSO and 10% DMF. Exposure to 100% DMSO is not recommended. Unfortunately, the compatibility of these devices with 100% DMF has not been determined.
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Can I order the filter only, coming with no tube part?
1 answer-
Unfortunately, no, this product is not available as a stand alone item. The tubes are available separately under the product UFC50VL96.
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Is the 0.5 ml 3KDa Amicon device (UFC5003) compatible with a solution containing 5% SDS (Sodium Dodecyl Sulfate)?
1 answer-
The Amicon® Ultra centrifugal devices are designed for processing biological fluids and aqueous solutions. Amicon® Ultra Filter Devices are compatible with concentrations of Sodium dodecyl sulfate (SDS)of 0.1% or less. Prior to use, it is important to verify the chemical compatibility of the sample with the device.
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Hi, Do these filters come with expiry dates? If so where would I be able to find them on the product?
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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How long should I centrifugate at 14.000 g and how long for the reverse spin at 1000 g?
1 answer-
Typical concentration time for the 3kD membrane device is approximately 30 minutes. However, spin times may need to be adjusted to either a shorter or longer time depending on the sample starting volume, initial concentration, composition, and desired minimum volume. The reverse spin is 1000g for 2 minutes. Please review the product user guide at the link below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/340/pr05485-rev0219.pdfHelpful?
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Hi, how many g's can be used for the ultracell-3K #UFC500396 product?
1 answer-
The recommended speed with a 35 degree fixed angle rotor is 14,000 g with a reverse spin of 1000 g. This information can be found at this link: https://www.sigmaaldrich.com/products/protein-biology/protein-sample-prep/amicon-centrifugals#ultracentrifugal
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