C7521

Human Collagen Type IV

from human placenta, powder, suitable for substrate for collagenase and for diagnostic assay manufacturing

• Sinónimos: Collagen Type 4
• Número CAS: 9007-34-5
• UNSPSC Code: 12352202
• NACRES: NA.54
• EC Number: 232-697-4
• MDL number: MFCD00130825

Propiedades

• Nombre del producto: Colágeno from human placenta, Bornstein and Traub Type IV, powder
• biological source: human placenta
• description: Sigma Type VI
• form: powder
• impurities: HIV, hepatitis B and hepatitis C, none detected
• solubility: 0.5 M acetic acid: 1 mg/mL
• suitability: suitable for substrate for collagenase
• UniProt accession no.: P08572; P29400; P53420; Q01955; Q14031
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Descripción

Other Notes

El colágeno se clasifica en una serie de distintos tipos desde el punto de vista estructural y genético. Nosotros utilizamos la nomenclatura propuesta por Bornstein y Traub. Tenga cuidado de no confundir las designaciones de tipo Sigma con los tipos de clasificación de colágeno reconocidos

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Biochem/physiol Actions

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.[1]

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)