Saltar al contenido
MilliporeSigma

R0278

Tampón RIPA

RIPA Buffer

Sinónimos:

Radioimmunoprecipitation assay buffer

Slide 1 of 1
Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

50 ML

$108.00

500 ML

$574.00

$108.00

Disponible para enviar HOYDetalles

Acerca de este artículo

UNSPSC Code:
12161703
NACRES:
NA.56
MDL number:
MFCD02100484

Documentos clave

Ver Toda la documentación

Saltar a

Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle

description

protease free

form

liquid

packaging

pkg of 50 mL
pkg of 500 mL

technique(s)

immunoprecipitation (IP): suitable

color

colorless

pH

7.8-8.2 (25 °C, 1 ×)

storage temp.

2-8°C

Quality Level

200

¿Está buscando productos similares? Visita Guía de comparación de productos

Categorías relacionadas

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 4

Este artículo
20-18811814389001T9285
Tampón RIPA

Sigma-Aldrich

R0278

Tampón RIPA

Tampón de lisis RIPA, X10 100 mL RIPA Lysis Buffer, 10X for Immunoprecipitation & Western Blotting.

Millipore

20-188

Tampón de lisis RIPA, X10

Red Blood Cell Lysis Buffer solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions

Roche

11814389001

Red Blood Cell Lysis Buffer

Tampón Tris-EDTA 100X concentrate for Northern and Southern blotting

Sigma-Aldrich

T9285

Tampón Tris-EDTA

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

200

form

liquid

form

liquid

form

solution

form

liquid

pH

7.8-8.2 (25 °C, 1 ×)

pH

-

pH

-

pH

7.8-8.2 (1 × in water)

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

room temp

color

colorless

color

-

color

-

color

-

Application

El tampón RIPA se ha utilizado como reactivo para lisis celular en los siguientes estudios experimentales:
  • Fenotipado profundo del tejido periférico basado en fibroblastos, que facilita la estratificación mecanicisita de la enfermedad de Parkinson esporádica (sPD).[1]
  • Demostración de la inhibición de la fosforilación oxidativa (OXPHOS), que puede activar la necroptosis dependiente de la proteína de tipo dominio de cinasa de linaje mixto (MLKL) en las células epiteliales pulmonares humanas.[2]

La disolución tampón RIPA permite la lisis celular y la solubilización proteica eficaces a la vez que evita la degradación de las proteínas y la interferencia en la actividad biológica y la inmunorreactividad de las proteínas. La disolución tampón RIPA también produce un bajo ruido de fondo en los análisis de inmunoprecipitación y los ensayos de pull-down molecular. Compatible con los geles de afinidad EZview™.

Features and Benefits

1 ml del tampón RIPA se utiliza para lisar células de una placa de cultivo de 100 mm (0,5 a 5 * 107 células) de la mayoría de líneas celulares adherentes de mamíferos.
Minimiza las interacciones proteicas inespecíficas para asegurar bajo ruido de fondo.

General description

EL tampón RIPA (ensayo de radioinmunoprecipitación) se suministra como una disolución lista para usar que no requiere preparación. Reduce al mínimo las interacciones inespecíficas de unión a proteínas para mantener el fondo bajo, al tiempo que permite las interacciones más específicas, lo cual hace posible estudiar las interacciones proteína-proteína relevantes. Es muy utilizado en aplicaciones como la inmunoprecipitación, ya que los componentes de este tampón no afectan de manera negativa a la mayoría de los anticuerpos y antígenos proteicos.
La disolución amortiguadora para análisis de radioinmunoprecipitación (tampón de RIPA) es un tampón de lisis utilizado para la lisis y la solubilización rápidas y eficientes de proteínas de células de mamífero cultivadas en suspensión y adheridas.

Other Notes

Disolución lista para utilizar que contiene NaCl 150 mM, IGEPAL® CA-630, al 1,0 %, desoxicolato sódico al 0,5 %, SDS al 0,1%, Tris 50 mM, pH 8.0.

Legal Information

EZview is a trademark of Sigma-Aldrich Co. LLC
IGEPAL is a registered trademark of Solvay

Related product

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

890890P

18:1 TAP (DOTAP), Avanti Research™ - A Croda Brand

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Clase de almacenamiento

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000504725
0000504726
0000504726
0000504725
0000437799

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

Busque por un COA

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Elham Abbasloo et al.
Neuropeptides, 60, 7-12 (2016-08-21)
Pain represents a major contributing factor to the individual's quality of life. Although pain killers as opioids, endogenous or exogenous peptides can decrease pain perception, the chronic use of them leads to antinociceptive tolerance. It has been demonstrated that neuropeptide
Hartmut Jahns et al.
Nature communications, 6, 6317-6317 (2015-03-07)
An established means of improving the pharmacokinetics properties of oligoribonucleotides (ORNs) is to exchange their phosphodiester linkages for phosphorothioates (PSs). However, this strategy has not been pursued for small interfering RNAs (siRNAs), possibly because of sporadic reports that PS siRNAs
Zuzana Keckesova et al.
Nature, 543(7647), 681-686 (2017-03-23)
Post-mitotic, differentiated cells exhibit a variety of characteristics that contrast with those of actively growing neoplastic cells, such as the expression of cell-cycle inhibitors and differentiation factors. We hypothesized that the gene expression profiles of these differentiated cells could reveal
Impaired oxidative phosphorylation regulates necroptosis in human lung epithelial cells
Koo MJ, et al.
Biochemical and Biophysical Research Communications, 464(3), 875-880 (2015)
Paula Kinsella et al.
Investigational new drugs, 34(2), 159-167 (2016-02-18)
The C-KIT receptor tyrosine kinase is constitutively activated in the majority of gastrointestinal stromal tumours (GIST). Imatinib (IM) a selective inhibitor of C-KIT, is indicated for the treatment of KIT-positive unresectable and/or metastatic GIST, and has tripled the survival time
Ver todos los artículos relacionados

Artículos

Insulin and Dexamethasone in Cell Culture

Discover cell culture applications for hormones including insulin and dexamethasone. Explore high-quality supplements.

Contenido relacionado

Protein Research Guide

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

GC5, GC10, and Thunderbolt Competent Cells
Product Information Sheet - R0278

Instructions

CelLytic Protein Extraction Reagents
1 Review This action will navigate to reviews.

Questions

1–10 of 12 Questions  

  1. we bought a bottle of RIPA buffer and want to make alliquots of this bottle. Can we store the alliquots of the buffer by -20? Or is it better to keep them between 2°C-8°C ?

    1 answer

    1. It is recommended to store the product at 2–8C. Studies have not been performed on aliquots and to determine if refrigerated storage will extend the shelf life of this product. We would recommend searching the general research literature or contacting researchers who routinely use this product to see if solution stability information is available. If a recommended storage is not given in the literature, it is usually best to make solutions fresh for use. If you decide to store the aliquots at -20°C, we would recommend validating activity of the supplemented buffer after freezing and avoid any thawing/freezing cycle. Please refer to this technical bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  2. Is this RIPA lysis buffer capable of extracting nuclear protein in mammalian cells?

    1 answer

    1. Yes, RIPA buffer typically lyses cell nuclei. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  3. Can I use R0278 for human protein extract for lymphocytes?

    1 answer

    1. This product has not been tested for protein extract from human lymphocytes. References using RIPA buffer with human lymphocytes can be found here:
      https://www.sciencedirect.com/science/article/pii/S0021925818639675
      https://www.sciencedirect.com/science/article/pii/S000887491530037X?via%3Dihub

      Helpful?

  4. Can I use the Ripa Buffer to extract yeast protein and make the Bradford assay on it?

    1 answer

    1. RIPA (Radio-Immunoprecipitation Assay) Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. For more information, please see the Technical Bulletin: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/350/775/r0278bul.pdf

      Helpful?

  5. How long can the RIPA buffer be stored in 4C? I stored it for almost 6 years, can I still use it?

    1 answer

    1. Regarding stability in the recommended temperature of 2-8 °C, this product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  6. Can this be used for Western blot experiments?

    1 answer

    1. Yes, this product would be suitable for western blotting.

      Helpful?

  7. Can Product R0278, RIPA buffer, be used on tissue samples, or only on cultured cells?

    1 answer

    1. R0278 can be used on tissue, if it is well homogenized.

      Helpful?

  8. How does Product R0278, RIPA buffer, compare to Product No. C2978 (CelLytic M)?

    1 answer

    1. RIPA buffer contains SDS, which is considered to be a harsh detergent; it can denature many proteins. C2978 is a buffered solution (pH 7.6) that contains a mild detergent; it is non-denaturing for most proteins.  The exact formulation of C2978, however, is proprietary.

      Helpful?

  9. I left the bottle of Product R0278, RIPA buffer, on my bench for about a week. Can I still use it?

    1 answer

    1. Yes, you should be able to use this product.  Although we recommend storage in the refrigerator at 2-8°C for long-term storage, Product No. R0278 may be kept at ambient temperature (our normal shipping condition) for a short time (approximately 2 weeks).

      Helpful?

  10. Does Product R0278, RIPA buffer, contain any protease inhibitors or phosphatase inhibitors?

    1 answer

    1. This buffer does not contain any protease or phosphatase inhibitors, however, they may be added to insure against possible proteolysis.

      Helpful?

1–10 of 12 Questions  

Reviews

Active Filters

  1. 1 Ratings-Only Review

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico