BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71.

BPR-3P0128 inhibits RNA-dependent RNA polymerase elongation and VPg uridylylation activities of Enterovirus 71.

Antiviral research (2014-12-03)

Arul Balaji Velu, Guang-Wu Chen, Po-Ting Hsieh, Jim-Tong Horng, John Tsu-An Hsu, Hsing-Pang Hsieh, Tzu-Chun Chen, Kuo-Feng Weng, Shin-Ru Shih

PMID25448086

RESUMEN

Enterovirus 71 (EV71) infections can cause hand, foot, and mouth disease with severe neurological complications. Because no clinical drug is available for treating EV71 infections, developing an efficient antiviral medication against EV71 infection is crucial. This study indicated that 6-bromo-2-[1-(2,5-dimethylphenyl)-5-methyl-1H-pyrazol-4-yl] quinoline-4-carboxylic acid (BPR-3P0128) exhibits excellent antiviral activity against EV71 (EC50 = 0.0029 μM). BPR-3P0128 inhibits viral replication during the early post infection stage, targets EV71 RNA-dependent RNA polymerase and VPg uridylylation, and also reduces viral RNA accumulation levels and inhibits viral replication of EV71.

MATERIALES

Número de producto

Marca

Descripción del producto

L3771

Sigma-Aldrich

Dodecilsulfatosódico, BioReagent, suitable for electrophoresis, Molecular Biology, ≥98.5% (GC)

75746

Sigma-Aldrich

Dodecilsulfatosódico, ≥99.0% (GC), dust-free pellets

S3014

Sigma-Aldrich

Cloruro de sodio, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)

S5150

Sigma-Aldrich

Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology, suitable for cell culture

S7653

Sigma-Aldrich

Cloruro de sodio, BioXtra, ≥99.5% (AT)

S5886

Sigma-Aldrich

Cloruro de sodio, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

S8776

Sigma-Aldrich

Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture

436143

Sigma-Aldrich

Dodecilsulfatosódico, ACS reagent, ≥99.0%

71736

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, Molecular Biology, 10% in H₂O

L4509

Sigma-Aldrich

Dodecilsulfatosódico, ReagentPlus^®, ≥98.5% (GC)

S6546

Sigma-Aldrich

Sodium chloride solution, 5 M

71725

Sigma-Aldrich

Dodecilsulfatosódico, BioUltra, Molecular Biology, ≥99.0% (GC)

05030

Sigma-Aldrich

Sodium dodecyl sulfate solution, BioUltra, 20% in H₂O

59222C

SAFC

Sodium chloride solution, 5 M

M0375

Sigma-Aldrich

Maleic acid, ReagentPlus^®, ≥99% (HPLC)

74255

Supelco

Dodecilsulfatosódico, dust-free pellets, suitable for electrophoresis, Molecular Biology, ≥99.0% (GC)

28-2270

Sigma-Aldrich

Cloruro de sodio, JIS special grade, ≥99.5%

P9625

Sigma-Aldrich

Phenazine methosulfate

71726

Supelco

Dodecilsulfatosódico, suitable for ion pair chromatography, LiChropur^™, ≥99.0%

71376

Sigma-Aldrich

Cloruro de sodio, BioUltra, Molecular Biology, ≥99.5% (AT)

S1679

Sigma-Aldrich

Cloruro de sodio, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%

71386

Sigma-Aldrich

Sodium chloride solution, BioUltra, Molecular Biology, ~5 M in H₂O

204439

Sigma-Aldrich

Cloruro de sodio, 99.999% trace metals basis

PHR1321

Supelco

Cloruro de sodio, Pharmaceutical Secondary Standard; Certified Reference Material

L6026

Sigma-Aldrich

Dodecilsulfatosódico, BioXtra, ≥99.0% (GC)

92816

Supelco

Maleic acid, qNMR standard, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

71729

Sigma-Aldrich

Dodecilsulfatosódico, ≥98.0% (GC)

71387

Supelco

Cloruro de sodio, reference material for titrimetry, certified by BAM, >99.5%

L5750

Sigma-Aldrich

Dodecilsulfatosódico, 92.5-100.5% based on total alkyl sulfate content basis

PHR1272

Supelco

Maleic acid, Pharmaceutical Secondary Standard; Certified Reference Material