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Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in goat

Name: Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in goat
Brand: SIGMA
Price: 282 USD
Availability: InStock

affinity isolated antibody, buffered aqueous solution

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1 mL	Check Cart for Availability	$282.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

MDL number:

MFCD00162368

Conjugate:

peroxidase conjugate

Clone:

polyclonal

Application:

ELISA (d)

Citations:

$282.00

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Properties

biological source

goat

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

IgG consists of four sub-classes, like IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL).

Immunogen

Purified guinea pig IgG

Application

Anti-H. pylori IgGs in guinea pig sera was measured by ELISA using HRP-conjugated goat anti-guinea pig IgG (A 7289) at a dilution of 1:2000 in 1% BSA/PBS for 1 hour at 37°C.

Anti-Guinea Pig IgG (whole molecule)-Peroxidase antibody is suitable for use in immunocytochemistry and ELISA.

Biochem/physiol Actions

Guinea pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-guinea pig IgGs conjugated to detectable substrates are useful tools for the analysis of target proteins. Anti-Guinea Pig IgG (whole molecule)-Peroxidase antibody is specific for guinea pig IgG.

IgGs are glycoprotein antibodies that modulate several immune responses. It plays a major role in providing immunity to the infant from the mother via the placenta. IgG activates the complement system and regulates the antibody-dependent cell-mediated cytotoxicity (ADCC).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Antiserum is developed in goat using purified guinea pig IgG as the immunogen. Antibody is isolated from goat anti-guinea pig IgG antiserum by immunospecific purification which removes essentially all goat serum proteins, including immunoglobulins that do not specifically bind to guinea pig IgG. Goat anti-guinea pig IgG is conjugated to Sigma Horseradish Peroxidase, Type VI (Product No. P 8375) by a modification of the periodate method of Wilson and Nakane.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	A5670	A6917	A4174
Sigma-Aldrich A7289 Anti-Guinea Pig IgG (whole molecule)−Peroxidase antibody produced in goat	Sigma-Aldrich A5670 Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A6917 Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit	Sigma-Aldrich A4174 Anti-Goat IgG (whole molecule)–Peroxidase antibody produced in rabbit
description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution	description affinity isolated antibody, buffered aqueous solution
conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate	conjugate peroxidase conjugate
technique(s) direct ELISA: 1:10,000	technique(s) dot blot: 1:150,000 (chemiluminescent), direct ELISA: 1:40,000	technique(s) direct ELISA: 1:10,000	technique(s) direct ELISA: 1:10,000
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
biological source goat	biological source rabbit	biological source rabbit	biological source rabbit
clone polyclonal	clone polyclonal	clone polyclonal	clone polyclonal
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Pictograms

GHS07,GHS09

Signal Word

Warning

Hazard Codes

H315,H317,H319,H411

Precautionary Statements

P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 3

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