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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $228.00 |
About This Item
$228.00
usage
sufficient for 70 purifications
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
: <30 min kit run time, sample volume: 1-5 mL
greener alternative category
, Aligned
storage temp.
15-25°C
General description
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
Application
Features and Benefits
- Purified plasmid DNA in less than 30 minutes
- Purify up to 15 μg of plasmid DNA
- No detectable genomic DNA or RNA contamination
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable | technique(s) DNA extraction: suitable | technique(s) DNA purification: suitable |
| usage sufficient for 70 purifications | usage sufficient for 70 preparations | usage sufficient for 350 preparations | usage sufficient for 350 purifications |
| test parameters : <30 min kit run time, sample volume: 1-5 mL | test parameters - | test parameters : <30 min kit run time, sample volume: 1-5 mL bacterial culture | test parameters : <30 min kit run time, sample volume: 1-5 mL bacterial culture |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| greener alternative category , Aligned | greener alternative category | greener alternative category | greener alternative category |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 2
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