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Anti-Rabbit IgG, Native antibody, Mouse monoclonal

Name: Anti-Rabbit IgG, Native antibody, Mouse monoclonal
Brand: SIGMA
Price: 471 USD
Availability: InStock

clone RabT-50, purified from hybridoma cell culture

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Size/SKU	Availability	Price
200 μL	Available to ship TODAYfromMILWAUKEE	$455.00 $471.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

unconjugated

Clone:

RabT-50, monoclonal

Application:

ELISA (i), ICC, WB

Citations:

$471.00

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Properties

biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

RabT-50, monoclonal

form

buffered aqueous solution

species reactivity

rabbit

should not react with

human, rat, chicken, cat, bovine, monkey, goat, pig, horse

concentration

~1 mg/mL

technique(s)

immunocytochemistry: suitable, indirect ELISA: suitable, western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Description

General description

Anti-Rabbit IgG, Native antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma RabT-50 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Immunoglobulin G (IgG) is found abundantly in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy and the light chains are linked at VH and CH1 domain to form the Fragment antigen binding (Fab) arm. The antigen binds to the V regions of the antibody.

This antibody reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG.

Immunogen

Enriched fraction of rabbit IgG light chain.

Application

Anti-Rabbit IgG, Native antibody, Mouse monoclonal has been used in:

immunoblotting
immunocytochemistry
immunohistochemistry
enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Anti-Rabbit IgG, Native antibody, Mouse monoclonal reacts specifically with non-reduced rabbit IgG and does not react with reduced rabbit IgG. It does not recognize human, monkey, bovine, horse, pig, goat, cat, rat, and chicken IgGs.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	R3155	AB21	AP164
Sigma-Aldrich R2655 Anti-Rabbit IgG, Native antibody, Mouse monoclonal	Sigma-Aldrich R3155 Anti-Rabbit IgG, Native−Peroxidase antibody, Mouse monoclonal	Sigma-Aldrich AB21 Goat Anti-Rabbit IgG	Sigma-Aldrich AP164 Rabbit Anti-Rat IgG Antibody
description clone RabT-50, purified from hybridoma cell culture	description clone RabT-50, purified from hybridoma cell culture	description -	description Chemicon^®, from rabbit
biological source mouse	biological source mouse	biological source -	biological source rabbit
technique(s) immunocytochemistry: suitable, western blot: 1-2 μg/mL using 10 ng of non-reduced rabbit IgG (Cat. No. I5006) per lane, indirect ELISA: suitable	technique(s) immunocytochemistry: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.	technique(s) immunoprecipitation (IP): suitable	technique(s) western blot: suitable, immunoprecipitation (IP): suitable, ELISA: suitable
conjugate unconjugated	conjugate peroxidase conjugate	conjugate -	conjugate unconjugated
species reactivity rabbit	species reactivity rabbit	species reactivity rabbit	species reactivity rat
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form serum	antibody form affinity isolated antibody
isotype IgG1	isotype IgG1	isotype -	isotype -

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Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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