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RAB0444

Cell-Based ELISA for detecting phospho-STAT3 (pTyr705) in cultured cell lines

96 assays

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1 kit
Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
$593.00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... STAT3(6774)
mouse ... STAT3(20848)
rat ... STAT3(25125)
Species reactivity:
rat, human, mouse
Storage temp.:
−20°C
Shipped in:
wet ice

$593.00

Usually ships in 1 week. (Orders outside of US and Europe, please allow an additional 1-2 weeks for delivery)
​
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usage

96 assays

species reactivity

rat, human, mouse

technique(s)

ELISA: suitable, capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... STAT3(6774)
mouse ... STAT3(20848)
rat ... STAT3(25125)

General description

The Cell-Based Stat3 (pTyr705) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat3 (pTyr705) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.
The STAT3 (signal transducer and activator of transcription)gene is mapped to human chromosome 17q21.2. Upon phosphorylation, STAT3 translocates to nucleus from cytoplasm, where it mediates a number of biological processes. The protein structure includes the following domains : N-domain, coiled-coil domain, DNA-binding domain, linker domain, SH2 domain and transcriptional activation domain.

Immunogen

Stat3 (pTyr705) synthetic phosphopeptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Signal transducer and activator of transcription (STAT) proteins are a family of latent cytoplasmic transcription factors involved in cytokine, hormone, and growth factor signal transduction. STAT proteins mediate broadly diverse biological processes, including cell growth, differentiation, apoptosis, fetal development, transformation, inflammation, and immune response. Unlike all other members of the STAT gene family, ablation of STAT3 leads to embryonic lethality and it has been suggested that this protein might represent a primordial STAT protein. It evokes a number of distinct responses in different cells, including induction of an acute-phase response in hepatoma cells, stimulation of proliferation in B lymphocytes, activation of terminal differentiation and growth arrest in monocytes, and maintenance of the pluripotency of embryonic stem cells. Dysregulation of STAT3 signaling has been demonstrated to contribute to malignant cellular transformation. Src kinase-mediated activation of STAT3 has been shown to be essential in prostate and ovarian carcinomas. In head and neck cancers, constitutive STAT3 activity with up-regulated epidermal growth factor receptor (EGFR) signaling plays an important role in malignant proliferation. STAT3 may be a key player in the pathogenesis of diverse human cancers which makes this molecule a prime target for novel therapies.

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Cell-Based ELISA for detecting phospho-STAT3 (pTyr705) in cultured cell lines 96 assays

Sigma-Aldrich

RAB0444

Cell-Based ELISA for detecting phospho-STAT3 (pTyr705) in cultured cell lines

Phospho-Stat3 (pTyr705) and pan-Stat3 ELISA Kit for detection of phospho-stat3 (pTyr705) and pan-stat3 in human, mouse or rat cell and tissue lysates

Sigma-Aldrich

RAB0447

Phospho-Stat3 (pTyr705) and pan-Stat3 ELISA Kit

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines 96 assays

Sigma-Aldrich

RAB0448

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines 192 assays

Sigma-Aldrich

RAB0449

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines

description

96 assays

description

for detection of phospho-stat3 (pTyr705) and pan-stat3 in human, mouse or rat cell and tissue lysates

description

96 assays

description

192 assays

Gene Information

human ... STAT3(6774)
mouse ... STAT3(20848)
rat ... STAT3(25125)

Gene Information

human ... STAT3(6774)
mouse ... STAT3(20848)
rat ... STAT3(25125)

Gene Information

human ... STAT4(6775)
mouse ... STAT4(20849)

Gene Information

human ... STAT4(6775)
mouse ... STAT4(20849)

species reactivity

rat, human, mouse

species reactivity

human, mouse, rat

species reactivity

mouse, human

species reactivity

human, mouse

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

usage

96 assays

usage

-

usage

96 assays

usage

192 assays

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • RABBLOCK
    5x Blocking Solution (Item F)
    SDS
  • RABFIX1
    Fixing Solution (Item D)
    SDS
  • RABHRP1
    HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)
    SDS
  • RABHRP2
    HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)
    SDS
  • RABPLATE1
    Uncoated Microplate
    SDS
  • RABQUENCH
    Quenching Solution for Cell-based ELISA Assay (Item E)
    SDS
  • RABSTOP1
    Stop Solution
    SDS
See All (10)

Storage Class

10 - Combustible liquids

wgk

WGK 1

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