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WTA2

TransPlex^® Complete Whole Transcriptom Amplification Kit

Name: TransPlex<SUP>®</SUP> Complete Whole Transcriptom Amplification Kit
Brand: SIGMA

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

同義詞:

完整全转录组扩增试剂盒

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NACRES:

NA.55

UNSPSC Code:

41121800

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屬性

Quality Segment

200

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

描述

General description

经过优化的WTA2，可对福尔马林固定的石蜡包埋样品（FFPE）和其他受损或降解样本中的RNA进行扩增。全转录组扩增（WTA）技术可在4小时内对ng级的总RNA进行无3′偏差的代表性扩增。扩增产物适用于qPCR、微阵列分析和克隆等应用。WTA2试剂盒含有cDNA文库扩增所需的聚合酶。

Application

适于各种下游应用，包括：

qPCR
微阵列分析
克隆

完整全转录组扩增试剂盒用于：

建立过程控制深度测序同时分析猪粪便中DNA和RNA病毒的实验方案。[1]
反转录和cDNA扩增[2][3][4]
使用免疫捕获PPV颗粒释放的基因组RNA来合成和扩增cDNA库[5]
核酸制备和深度测序（提取的核酸随机引物用于cDNA合成）[6]

Biochem/physiol Actions

WTA2过程包括2个步骤。第一步，使用由拟随机3′端和通用5′端组成的非自身互补引物对样品RNA进行逆转录。在该过程中，被取代的单链将作为引物退火和延伸的新模板。所得OmniPlex cDNA文库由以通用末端序列为侧翼序列、含100-1000个碱基的随机重叠片段组成。第二步，使用WTA2聚合酶和通用末端引物对cDNA文库进行PCR扩增，生成WTA2产物。

Features and Benefits

在4小时内实现高达10,000x的扩增，并且手动操作时间低于30分钟
仅需20 pg的总RNA模板，即可扩增出适用于微阵列分析的cDNA
含有cDNA扩增所需的所有元件
实现对表达基因和外显子的线性扩增，无3′或5′偏差
有效扩增单细胞或小量RNA，包括来自动物、植物或微生物的mRNA和总RNA。

比較類似項目

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本產品	WGA2	SEQR	WTA1
Sigma-Aldrich WTA2 TransPlex^® Complete Whole Transcriptom Amplification Kit	Sigma-Aldrich WGA2 GenomePlex^®完整全基因组扩增（WGA）试剂盒	Sigma-Aldrich SEQR SeqPlex RNA扩增试剂盒	Sigma-Aldrich WTA1 TransPlex^® Whole Transcriptome Amplification Kit
technique(s) whole genome amplification: suitable	technique(s) whole genome amplification: suitable	technique(s) whole genome amplification: suitable	technique(s) whole genome amplification: suitable
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
dilution (WTA)	dilution (WGA)	dilution (WTA)	dilution (WTA)
input purified RNA	input purified DNA	input purified RNA	input purified DNA
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

套裝中的組件也可單獨購買

產品號碼

描述

SDS & 訂價

Library Synthesis Enzyme
SDS

Library Synthesis Solution
SDS

Amplification Mix
SDS

Library Synthesis Buffer
SDS

W4502
Water, Nuclease-Free Water, for Molecular Biology
SDS

Amplification Enzyme
SDS

D7295
Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent
SDS

存儲類別/等級

10 - Combustible liquids

wgk

WGK 3

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Questions

Anonymous

2 years ago

What are the differences between WTA1 and WTA2?

1 answer
1. Technical Support
  
  2 years ago
  
  WTA2 is designed to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Here are the main differences between the two kits:
  1. WTA1 was developed by Rubicon. WTA2 was developed by Sigma-Aldrich.
  2. The library synthesis primers for WTA2 are different and can prime more frequently, which is crucial for degraded RNAs.
  3. The library synthesis conditions are different due to the change in the primers.
  4. There are improved cycling conditions in WTA2 to optimize amplification of both high AT templates and high GC templates.
  5. WTA1 does not contain an amplification enzyme, which needs to be supplied separately. WTA2 includes the amplification enzyme.
  
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重要文件

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TransPlex^® Complete Whole Transcriptom Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias