LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5
Conditions | |
|---|---|
| column | Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U) |
| column temp. | 30 °C |
| mobile phase | [A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85) |
| flow rate | 0.2 mL/min |
| pressure | 1150 psi (79 bar) |
| sample | 10 ng/mL in mobile phase |
| injection | 1 µL |
| detector | MS, ESI(+), MRM mode |
Description | |
|---|---|
| Analysis Note | The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. |
| Categories | Analytical Chromatography, Immunosuppressants |
| Featured Industry | Vitamins, Nutraceuticals, and Natural Products |
| suitability | application for HPLC |
Materials
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