HPLC Columns

Browse our HPLC and UHPLC columns for greater separation process.

Supelco® HPLC and UHPLC columns meet today’s demands of fast U/HPLC, LC-MS, and biopolymer separation, as well as regulated pharmacopeia and agency methods within various industries. Supelco® HPLC products are available with Fused-Core® particle technology, monolithic silica, and fully porous particles, including ultra-pure silica. In addition, polymeric particles, alumina oxide, and zirconia particles are available for high pH stability.



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Superficially porous silica particles (SPP)/Fused Core® Technology

Superficially porous silica columns deliver higher speed and efficiency than fully porous particles of the same size. Fused-Core® HPLC columns like Ascentis® Express and BIOshell™ can convert any HPLC system into a fast HPLC workhorse with maximum speed and performance.

Monolithic silica

Monolithic silica-based Chromolith® HPLC columns allow rapid separations at very low column backpressure with high matrix tolerance and extended column lifetime, thus making them ideal products for high throughput and cost-efficient analyses of matrix rich samples. Chromolith® HPLC columns are made from monolithic silica with the bimodal pore structure having 2 µm macropores and 13 nm mesopores. This structure leads to various key advantages to the column.

Chromolith® WP column is a wide pore (300 Å) monolithic silica column made up of a single continuous rod of high purity, porous silica. This column has shown great potential compared to conventional columns with its consistent performance over a longer lifetime. In addition to various RP-phases a Protein A affinity column and an epoxy column, enabling customer specific modifications, are available.

Fully porous silica particles (FPP) Type B (high purity silica)

  • Discovery® HPLC columns are efficient for the analysis of biomolecules and small molecules. These columns have polar and non-polar bonding, with unique retention, selectivity, and no-bleed properties for LC-MS applications.
  • The Ascentis® bonded phases have a wide range of selectivity. Type B silica makes the column inert, stable, and reproducible. The high surface area of the silica is suitable for high, preparative loading capacity.
  • Purospher® STAR HPLC and UHPLC columns are best suited for the peak-tailing free separation of basic, neutral, and metal chelating samples. Their superior performance and stability allow maximum flexibility in method development with extended pH stability.
  • Titan™ UHPLC columns with 1.9 µm fully porous monodisperse silica, surpass other UHPLC columns with high performance at low cost. The columns are stable over a range of UHPLC flow and pressure conditions.
  • SeQuant® HILIC (hydrophilic interaction liquid chromatography) HPLC columns carry densely bonded, zwitterionic functional groups. These columns are available in a variety of lengths, particle sizes, and pore dimensions while showing an exceptional reproducibility and robustness.
  • Sepax® HPLC columns are highly porous silica-based columns mainly utilized for peptides and protein analysis.

Fully porous silica particles (FPP) Type A (conventional silica)

  • Supelcosil® silica-based HPLC column line has a wide range of bonded phase chemistries, with the particle sizes and column configurations ranging from microbore to preparative scale. These columns show great efficiency for polar, nonpolar, and charged analytes.
  • LiChrospher® and Superspher® HPLC columns are highly versatile and reliable, proven in thousands of established HPLC methods.
  • LiChrosorb® HPLC columns are packed with irregular silica particles ranging from analytical scale 5µm to large particles sized for production scale.
  • TSKgel®* HPLC and UHPLC columns are available for gel filtration, gel permeation, ion exchange, reversed-phase, hydrophobic interaction, hydrophilic interaction, and affinity chromatography.

Fully porous polymeric particles

  • apHera™ reversed-phase and amino HPLC columns are stable at the pH range 2-12 and are amenable to washing with alkaline solutions. They are ideal for the analysis of peptides, small proteins, carbohydrates, polar drugs, and organic acids.
  • Supelcogel® HPLC columns are packed with polystyrene-divinylbenzene co-polymer (PS-DVB) making them chemically stable. The two variations of PS-DVB co-polymer (6% and 8% cross-linked) maximize the separation process of the samples.
  • Hamilton Polymeric Reversed Phase HPLC columns are extremely inert, efficient, and durable, with high mechanical and thermal stability.
  • TSKgel®* HPLC and UHPLC columns are available for the separation of proteins, peptides, and other biomolecules. The columns containing silica and methacrylate particles with different pore sizes can accommodate analytes with a wide range of molecular weights. TSKgel®* HPLC and UHPLC columns are available for the separation of proteins, peptides, and other biomolecules. The columns containing silica and methacrylate particles with different pore sizes can accommodate analytes with a wide range of molecular weights.

Chiral HPLC Columns

We have a wide range of chiral HPLC columns for various applications.

  • Astec CYCLOBOND™ Chiral columns have bonded cyclodextrin phases that allow selective chemical separation of organic molecules.
  • Astec CHIROBIOTIC™ Chiral columns are based on macrocyclic glycoproteins bonded to high purity silica gels available for LC-MS chiral applications.
  • Astec Cellulose DMP Chiral columns with spherical, high-purity porous silica coated with DMPC (3,5-dimethylphenyl carbamate); can separate a wide range of chiral compounds under normal phase, polar organic, and SFC conditions.
  • Astec Copper Ligand Exchange Chiral columns are beneficial for resolving hydroxy acids, amino acids, bi-functional racemates, and other amines.
  • Protein-based Chiral columns utilize natural proteins immobilized onto silica support to provide enantiomeric selectivity.
  • Astec Polycyclic Amine Polymer Chiral columns offer a bonding technology, mainly used in the normal phase, for the separation of racemic compounds.




Highlights

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Webinar: Advances in Liquid Chromatography Separations Employing Carbon Particles

This presentation focuses on recent advances, in the past two years, in 'carbon-HPLC' in the analysis of compounds classically associated with being difficult to separate by conventional 'silica-HPLC.'

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Webinar: Supel™ Carbon LC Columns: New Tools for the Chromatographer’s Tool Box

Learn about porous graphitic carbon, a stationary phase material that is fully porous, carbon-based material—it is capable of analyzing small molecules and smaller proteins and peptides.

tips-and-tricks-to-improve-ms-sensitivity.jpg
Webinar: Tips & Tricks to Improve MS sensitivity

In this webcast, the origins and problems in LC–MS arising from impurities and contaminations will be described in detail and tips and tricks will be provided in order to enable or maintain high quality and high sensitivity LC-MS analyses.

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Webinar: Improving Bioanalysis Workflows

Quantitation of monoclonal antibodies by LC-MS is a difficult analytical challenge due to the complexity in the workflow, from sample prep to LC-MS quantitation. This presentation will highlight a simple and high throughput method for the quantitation of monoclonal antibodies and proteins in human and animal serum.


*Tosoh Bioscience columns are available in the following countries:

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