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Merck

16-316

Anti-Phosphotyrosine Antibody, 4G10® Platinum, HRP Conjugate

Upstate®, from mouse, peroxidase conjugate

Synonym(e):

phosphotyrosine

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100 μL

€ 783,00

€ 783,00


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Über diesen Artikel

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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Biologische Quelle

mouse

Qualitätsniveau

Konjugat

peroxidase conjugate

Antikörper-Produkttyp

primary antibodies

Klon

4G10®, monoclonal
PY20, monoclonal

Speziesreaktivität (Voraussage durch Homologie)

all

Hersteller/Markenname

Upstate®

Methode(n)

ELISA: suitable
western blot: suitable

Isotyp

IgG2b

Versandbedingung

wet ice

Posttranslationale Modifikation Target

phosphorylation (pTyr)

Angaben zum Gen

human ... PID1(55022)

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Dieser Artikel
05-1050X05-1050-M16-638
Gene Information

human ... PID1(55022)

Gene Information

-

Gene Information

-

Gene Information

human ... PID1(55022)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

4G10®, monoclonal, PY20, monoclonal

clone

4G10, monoclonal

clone

4G10®, monoclonal

clone

4G10®, monoclonal

conjugate

peroxidase conjugate

conjugate

-

conjugate

-

conjugate

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

wet ice

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Allgemeine Beschreibung

The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone.
Varies

Anwendung

Detect Phosphotyrosine with Anti-Phosphotyrosine Antibody, 4G10 Platinum, HRP Conjugate (Mouse Monoclonal Antibody), that has been shown to work in WB, ELISA.

Physikalische Form

100 μL of a proprietary mixture of protein G purified mouse monoclonal 4G10 (IgG2bƙ) HRP conjugate and protein A purified mouse monoclonal PY20, HRP conjugate (IgG2b) in PBS containing 0.05% Kathon and HRP stabilizing compounds. Sufficient for a minumum of 10 immunoblots, if used at the optimum concentration of 1:2000 – 1:4000 in 10 mL blocking buffer. Liquid.

Hinweis zur Analyse

Routinely evaluated by western blot.

Rechtliche Hinweise

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Gefahreneinstufungen

Aquatic Chronic 2 - Skin Sens. 1

WGK

WGK 3

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Ondřej Ballek et al.
Immunology letters, 142(1-2), 64-74 (2012-01-28)
Lck is the principal signal-generating tyrosine kinase of the T cell activation mechanism. We have previously demonstrated that induced Lck activation outside of lipid rafts (LR) results in the rapid translocation of a fraction of Lck to LR. While this
T Wang et al.
Nihon Rai Gakkai zasshi, 60(3-4), 146-151 (1991-07-01)
A comparative study on the usefulness of Kawatsu's silver impregnating staining method compared with S-100 protein ABC technique for the differential diagnosis of tuberculoid leprosy was carried out. The results of neurohistological examination obtained from both methods were almost the
Irmgard Hofmann et al.
The Journal of pharmacology and experimental therapeutics (2022-10-15)
VEGF and angiopoietin-2 (ANG2) have complementary roles in angiogenesis and promote an immunosuppressive tumor microenvironment. It is anticipated that combination of VEGF and ANG2 blockade could provide superior activity to blockade of either pathway alone, and that addition of VEGF/ANG2
Guillermina M Goñi et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(31), E3177-E3186 (2014-07-23)
Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase (NRTK) with key roles in integrating growth and cell matrix adhesion signals, and FAK is a major driver of invasion and metastasis in cancer. Cell adhesion via integrin receptors is well
Kogulan Yoganathan et al.
Frontiers in immunology, 13, 848577-848577 (2022-08-23)
The E protein transcription factors E2A and HEB are critical for many developmental processes, including T cell development. We have shown that the Tcf12 locus gives rise to two distinct HEB proteins, with alternative (HEBAlt) and canonical (HEBCan) N-terminal domains

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