386040

Anti-Hsp90 Mouse mAb (AC88)

liquid, clone AC88, Calbiochem^®

• Synonym(e): Anti-Hsp82, Anti-Hsp85, Anti-Hsp89
• UNSPSC-Code: 12352203
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: AC88, monoclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität (Voraussage durch Homologie): all
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (1 µg/mL, colorimetric); Immunoprecipitation ; Immunocytochemistry )
• Isotyp: IgG1
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HSP90AA1(3320)

Beschreibung

Allgemeine Beschreibung

Protein G purified mouse monoclonal antibody. Recognizes the ~90 kDa Hsp90 protein.

Recognizes the ~90 kDa Hsp90 protein.

This Anti-Hsp90 Mouse mAb (AC88) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Hsp90.

Immunogen

Achyla ambisexualis

Hsp90 isolated from water mold, Achyla ambisexualis

Anwendung

Immunoblotting (1 µg/ml, colorimetric)

Immunoprecipitation (see comments)

Immunocytochemistry (see comments)

Verpackung

Please refer to vial label for lot-specific concentration.

Physikalische Form

In 10 mM PBS, 50% glycerol, pH 7.2.

Angaben zur Herstellung

Following initial thaw, aliquot and freeze (-20°C).

Hinweis zur Analyse

Positive Control
Heat shocked HeLa cells, Heat shocked 3T3 cells, Heat shocked PC-12 cells, Heat shocked CHO-K1, Heat shocked Rat-2 cells

Sonstige Hinweise

Cross-reacts with glucocorticoid receptor in rodent. By immunoprecipitation, the antibody favors free Hsp90 over complexed Hsp90. The optimal dilution for immunoprecipitation is assay dependent and must be determined for each sample. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Harry, J.L. et al. 1990. Development190, 305.
Minoo, P. et al. 1989 J. Cell. Biol.109, 1937.
Riehl, R.M., et al. 1985. Biochemistry 24, 6586.
Sanchez, E.R., et al. 1985. J. Biol. Chem. 260, 12398.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable