487664
p-Nitrophenyl Phosphate Substrate Buffer
This p-Nitrophenyl Phosphate Substrate Buffer is validated for use in ELISA.
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 200,00 |
Über diesen Artikel
Quality Segment
sterility
non-sterile
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
dilution
(ELISA )
color
clearlight yellow
shipped in
wet ice
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Alkaline phosphatase substrate buffer
Other Notes
Suggested procedure for use of p-NPP in alkaline phosphatase-based ELISAs:
1. To 39 parts distilled water add 1 part 50X p-NPP Concentrate (Cat. No. 487663) and 10 parts p-NPP Substrate Buffer. Mix well and store protected from light. This diluted reagent is suitable for up to 10 h after preparation.
2. Complete all required incubations with antibodies and alkaline phosphatase-labeled probes.
3. Wash plate wells at least 4 times with Tris-buffered saline (TBS) containing 0.1% Tween®-20 detergent. DO NOT USE PHOSPHATE BUFFERS; inorganic phosphate is a potent inhibitor of alkaline phosphatase.
4. After the final wash, shake and blot all residual buffer from the wells.
5. Add 100 µl of 1X p-NPP Substrate Solution to each well and incubate for 15-60 min. (If a kinetic study is desired, readings at 405 nm may be taken at intermediate intervals).
[Note: Optimal incubation times may vary depending on the amount of enzyme present. If color develops too quickly, dilution of the probe, antibody, or alkaline phophatase-labeled reagent may be required.]
6. Stop the reaction by adding 100 µl of 1 N NaOH.
7. Read absorbance at 405 nm.
Note: Variations in time, reagent volumes, and temperature may require further standardization by the user.
Legal Information
Disclaimer
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Dieser Artikel | |||
|---|---|---|---|
| description This p-Nitrophenyl Phosphate Substrate Buffer is validated for use in ELISA. | description This p-Nitrophenyl Phosphate is validated for use in ELISA. | description Contains the following non-detergent sulfobetaines: 5 g of NDSB-195, 25 g of NDSB-201, and 5 g of NDSB-256. | description The Phosphatase Inhibitor Cocktail Set V, 50X, Lyophilized controls the activity of Phosphatase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications. |
| form liquid | form liquid | form solid | form lyophilized solid |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level - |
| manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® | manufacturer/tradename Calbiochem® |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| storage condition protect from light, OK to freeze | storage condition protect from light, OK to freeze | storage condition desiccated (hygroscopic), OK to freeze | storage condition protect from light, desiccated (hygroscopic), OK to freeze |
| shipped in wet ice | shipped in ambient | shipped in ambient | shipped in ambient |
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Irrit. 2
Lagerklasse
12 - Non Combustible Liquids
WGK
WGK 2
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