ABE1379

Anti-F-box/WD repeat-containing protein 7 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Archipelago homolog, F-box/WD repeat-containing protein 7, hAgo, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): feline (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), horse (based on 100% sequence homology), canine (based on 100% sequence homology), gorilla (based on 100% sequence homology), porcine (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_060785
• UniProt-Hinterlegungsnummer: Q969H0
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FBXW7(55294)

Beschreibung

Allgemeine Beschreibung

F-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NFκB2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.

~70 kDa observed. 79.7 kDa (isoform 1; Archipelago alpha, FBW7alpha, 110K, common), 70.3 kDa (isoform 2; Archipelago beta, FBW7beta, 69K), and 66.1 kDa (isoform 3; Archipelago gamma, FBW7gamma, Hippocampal) calculated.

Immunogen

Epitope: Internal

KLH-conjugated linear peptide corresponding to human F-box/WD repeat-containing protein 7.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected F-box/WD repeat-containing protein 7 in human cerebellum and human pancreas tissue.

Research Sub Category
Nuclear Receptors

This Anti-F-box/WD repeat-containing protein 7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of F-box/WD repeat-containing protein 7.

Biochem./physiol. Wirkung

Although immunogen sequence is present in all three spliced isoforms, both target band size by Western blotting and cytoplasmic staining pattern by immunohistochemistry are consistent with the detection of isoform 2 (Archipelago beta, FBW7beta, 69K) by this polyclonal antibody.

Physikalische Form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Angaben zur Herstellung

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Evaluated by Western Blotting in MCF-10A human breast epithelial cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected F-box/WD repeat-containing protein 7 in 10 µg of human breast cancer MDA-MB-231 and non-tumorigenic MCF-10A breast epithelial cell lysates.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable