MAB1049

Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2

clone 3H2, Chemicon^®, from mouse

• Synonym(e): BMP-4, DVR-4
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 3H2, monoclonal
• Speziesreaktivität: rat, human, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• Aufnahme: sample type neural stem cell(s); sample type mesenchymal stem cell(s)
• Isotyp: IgG2b
• NCBI-Hinterlegungsnummer: NM_001202.3; NM_130850.2; NM_130851.2
• UniProt-Hinterlegungsnummer: P12644
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... BMP4(652) ; mouse ... Bmp4(12159) ; rat ... Bmp4(25296)

Beschreibung

Allgemeine Beschreibung

17 kDa

Bone morphogenetic protein 4 (BMP4) is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.

Immunogen

Recombinant mouse BMP-4 (Masuhara, 1995).

Anwendung

Research Category
Stammzellforschung

Research Sub Category
Mesenchymale Stammzellen

Detect Bone Morphogenetic Protein 4 using this Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2 validated for use in ELISA, IC & WB.

Immunocytochemistry using osteoinductive cells; i.e., BFP and Saos-2

Western blot using osteoinductive cells; i.e., BFP and Saos-2

Optimal working dilutions must be determined by the end user.

Biochem./physiol. Wirkung

Specific for bone morphogenetic protein-4 (BMP-4) by Western blot and ELISA. No cross-reactivity with human BMP-2 or human TGF-β1. The antibody reacts with both human and mouse BMP-4 in both reduced and nonreduced conditions.
Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa).

Physikalische Form

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Angaben zur Herstellung

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Mouse brain extract

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable