Here's the recommended Coating Procedure:
Add 5 ml of sterile 0.01 N HCl solution to the VitroCol® serum vial containing 15 mg.
Gently mix contents until material is completely solubilized. It may be necessary to agitate at 2 to 10°C overnight.
Transfer the desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to the desired concentration using sterile 0.01 N HCl solution. A typical working concentration may range from 50 to 100 ug/ml.
Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
Add an appropriate amount of diluted VitroCol® material to the culture surface.
Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until the surface is dry.
After incubation, aspirate any remaining material.
Rinse coated surfaces carefully with sterile medium or PBS, avoiding scratching surfaces.
Coated surfaces are ready for use. They may also be stored at 2-10°C, damp or air dried if sterility is maintained.
Fortfahren mit
5008
Human Collagen Type I & III
from neo-natal fibroblast cells, 97% Type I with the remainder of Type III collagen, powder, suitable for cell culture, VitroCol®
Synonym(e):
Collagen Solution
Größe auswählen
| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
15 mg | Warenkorb auf Verfügbarkeit prüfen | € 449,00 |
Über diesen Artikel
Produktname
VitroCol®, Collagen from Human neonatal fibroblasts, 97% Type I with the remainder of Type III collagen, suitable for cell culture
biological source
human
sterility
sterile-filtered
form
lyophilized
packaging
pkg of 15 mg
manufacturer/tradename
Advanced BioMatrix
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/mL Endotoxin level
shipped in
wet ice
storage temp.
−20°C
General description
VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.
VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.
Preparation Note
Legal Information
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description Collagen from Human neonatal fibroblasts, 97% Type I with the remainder of Type III collagen, suitable for cell culture | description from human, pack of 0.25 mg | description 3D cell culture kit includes: Human Collagen, Mediums (RPMI, M199 and DMEM), PBS with Phenol Red and Neutralization Solution | description from bovine, pack of 30 mg |
| sterility sterile-filtered | sterility - | sterility - | sterility - |
| biological source human | biological source human | biological source rat | biological source bovine |
| technique(s) cell culture | mammalian: suitable | technique(s) cell attachment: suitable | technique(s) cell culture | mammalian: suitable, cell based assay: suitable, activity assay: suitable | technique(s) cell attachment: suitable |
| form lyophilized | form liquid | form liquid | form liquid |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| storage temp. −20°C | storage temp. - | storage temp. 2-8°C | storage temp. - |
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Which is the working concentration if I intend to use it for flask coating?
1 answer-
Helpful?
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