76216

Papain aus Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

• CAS-Nummer: 9001-73-4
• UNSPSC Code: 12352204
• eCl@ss: 42030311
• EC Number: 232-627-2
• NACRES: NA.54
• MDL number: MFCD00131791
• EG-Nummer: 3.4.22.2 (BRENDA, IUBMB)
• Specific activity: ≥10 U/mg protein (~25 mg/ml)
• Biological source: papaya

Eigenschaften

• biological source: papaya
• Quality Level: 100
• form: solution
• specific activity: ≥10 U/mg protein (~25 mg/ml)
• technique(s): cell culture | mammalian: suitable, single cell analysis: suitable
• color: light brown
• storage temp.: 2-8°C

Beschreibung

General description

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.

Application

Papain wird als Fleischzartmacher eingesetzt. Es wird außerdem zur Auflösung von Geweben bei der Herstellung von Primärzellkulturen verwendet.

Papain from Carica papaya has been used:

• in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
• to study its effects on Alpaca seminal plasma viscosity and sperm function
• to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa[1]

Biochem/physiol Actions

Papain ist eine Cysteinprotease, die Peptidbindungen und bekanntermaßen auch das Fc-Fragment von Antikörpern spaltet.

Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.

Other Notes

1 U entspricht der Enzymmenge, die 1 μmol N-Benzoyl-L-arginin-ethylester (BAEE, Fluka^®-Nr. 12880) pro Minute bei pH 6.2 und 25°C hydrolysiert

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves