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A8592

Monoklonale ANTI-FLAG® M2-Peroxidase (HRP) in Maus hergestellte Antikörper

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(e):

Monoklonaler ANTI-FLAG® M2-Antikörper in Maus hergestellte Antikörper, Anti-ddddk, Anti-dykddddk

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Größe/SKUVerfügbarkeitPreis
0.2 mg
Warenkorb auf Verfügbarkeit prüfen
€ 730,00
1 mg
Warenkorb auf Verfügbarkeit prüfen
€ 1.470,00
5 x 1 mg
Warenkorb auf Verfügbarkeit prüfen
€ 1.980,00

Über diesen Artikel

NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
peroxidase conjugate
Clone:
M2, monoclonal
Application:
ELISA (i)
Citations:
1904

€ 730,00

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Unterstützung erhalten

biological source

mouse

Quality Segment

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPX1(2876)

General description

Die monoklonale Anti-FLAG M2-Peroxidase ist ein Maus-IgG-Antikörper, der kovalent an Meerrettichperoxidase (HRP) konjugiert ist. Der Antikörper bindet an FLAG Fusionsproteine und erkennt das FLAG Epitop an N-terminalen, Met-N-terminalen, C-terminalen und internen FLAG Peptiden.

Application

Empfohlene Verdünnung für Immunzytochemie und Western Blot 1:100 bis 1:1000
Empfohlene Verdünnung für ELISA 1:20.000

Weitere Informationen zu den Produkten finden Sie in unserem FLAG® Literatur-Portal.
Für die einfache Detektion in einem Schritt mittels Immunzytochemie, ELISA oder Western Blot. Besonders hilfreich für den Nachweis von FLAG-Fusionsproteinen, die im Wirtsorganismus Maus exprimiert werden, wo sekundäre Anti-Maus-Antikörper unter Umständen eine Kreuzreaktivität verursachen.

Physical form

Lösung in phosphatgepufferter Kochsalzlösung, enthält 50 % Glycerin plus Konservierungsmittel und Stabilisator.

Preparation Note

Die ANTI-FLAG M2-Peroxidaselösung in Tris-gepufferter Kochsalzlösung (TBS) verdünnen: 0,05 M Tris, pH 7,4, mit 0,15 M NaCl.

Other Notes

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Dieser Artikel
F1804SAB4200119F4049
Monoklonale ANTI-FLAG® M2-Peroxidase (HRP) in Maus hergestellte Antikörper clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

A8592

Monoklonale ANTI-FLAG® M2-Peroxidase (HRP) in Maus hergestellte Antikörper

Monoklonaler ANTI-FLAG® M2-Antikörper in Maus hergestellte Antikörper 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

F1804

Monoklonaler ANTI-FLAG® M2-Antikörper in Maus hergestellte Antikörper

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat 2-4 mg/mL, clone 6F7, purified immunoglobulin

Sigma-Aldrich

SAB4200119

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat

Monoklonales ANTI-FLAG®-M2-FITC in Maus hergestellte Antikörper clone M2, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

F4049

Monoklonales ANTI-FLAG®-M2-FITC in Maus hergestellte Antikörper

description

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

description

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

description

2-4 mg/mL, clone 6F7, purified immunoglobulin

description

clone M2, purified immunoglobulin, buffered aqueous solution

clone

M2, monoclonal

clone

M2, monoclonal

clone

6F7, monoclonal

clone

M2, monoclonal

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

FITC conjugate

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

rat

biological source

mouse

technique(s)

indirect ELISA: 1:20,000

technique(s)

-

technique(s)

western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

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signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

Lagerklasse

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

55965-84-9

CAS No.

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Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–7 of 7 Questions  

  1. I am currently using your product A8592 HRP in my analytical method with QuantaBlu fluorescence substrate complex. Is it possible to use TMB substrate instead of QuantaBlu fluorescence substrate with the A8592 HRP conjugate?

    1 answer

    1. A8592 is an HRP-conjugated antibody. The product information sheet can be downloaded here:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/306/a8592dat-mk.pdf

      Although we do not routinely test 3,3′,5,5′-tetramethylbenzidine (TMB) in-house, it is a standard chromogenic substrate for horseradish peroxidase, so it is expected to work with the A8592 conjugate.

      QuantaBlu from ThermoFisher is a fluorogenic substrate; TMB is colorimetric.
      You should expect lower sensitivity with TMB compared with QuantaBlu, as fluorescence detection generally offers higher sensitivity.
      The TMB-HRP reaction produces a soluble end-product that is blue in color and can be read spectrophotometrically at 370 nm or 650 nm.
      The reaction may be stopped with acid, resulting in a yellow solution that is read at 450 nm.

      Published protocol for use of A8592 together with TMB include:

      https://pmc.ncbi.nlm.nih.gov/articles/PMC3709174/pdf/cpb-0026-0363.pdf
      https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2022.869825/full
      https://www.nature.com/articles/s41556-024-01448-1

      Helpful?

  2. How is Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, supplied?

    1 answer

    1. Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative.  The conjugate protein concentration is approximately 1 mg/ml.

      Helpful?

  3. Can product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, be used for immunohistochemistry?

    1 answer

    1. The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What concentration of Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, is recommended for my application?

    1 answer

    1. An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot.  Minimum antibody titer recommended for ELISA is 1:20,000.  Adjust antibody titer to maximize detection sensitivity and to minimize background.

      Helpful?

  6. I am getting a high background on my Western blots using Product No. A8592, Monoclonal Anti-FLAG® M2-Peroxidase (HRP) antibody.  What can I do to fix this?

    1 answer

    1. There is a troubleshooting guide provided at the end of the product information sheet.  Some suggestions include the following:  adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures.

      Helpful?

  7. What are the appropriate storage conditions for Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody?

    1 answer

    1. This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C.  Once diluted, repeated freezing and thawing is not recommended.

      Helpful?

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