C1907

Calcineurin aus Rinderhirn

lyophilized powder, ≥2,500 units/mg protein

• Synonym(e): Calmodulin-bindendes Protein
• CAS-Nummer: 90371-51-0
• MDL-Nummer: MFCD00130697
• UNSPSC-Code: 51111800
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: bovine
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥2,500 units/mg protein
• Mol-Gew.: dimer ~77 kDa; subunit mol wt 19-58 kDa
• Zusammensetzung: Protein, 0.3-1.7% Lowry
• Löslichkeit: H₂O: soluble
• UniProt-Hinterlegungsnummer: P48452
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... PPP3CA(286852)

Beschreibung

Allgemeine Beschreibung

Calcineurin (CaN) comprises CaN A and CaN B subunits. It exists as a heterodimer with a calmodulin-binding domain, catalytic site, a CaN B binding domain, and an autoinhibitory domain.[1]

Anwendung

Calcineurin from bovine brain has been used:

• as a positive control in western blot analysis of oocytes and cumulus cells proteome[2]
• as a positive control in calmodulin (CaM)-agarose binding assay[3]
• to test its phosphatase activity in the presence of okadaic acid[4]

Biochem./physiol. Wirkung

Calcineurin (CaN) activity is stimulated by nickel (Nⁱ²⁺) and manganese (Mn²⁺) ions.[1] It participates in the coupling of Ca²⁺ signals.[1] CaN may regulate oocyte growth and meiotic maturation in porcine.[2] It also participates in gene regulation, cell survival, and death.[4]

Calcineurin is a cyclosporin-sensitive, calcium-regulated, serine-threonine protein phosphatase with broad substrate specificity. It is the major calmodulin-binding protein found in the brain. First identified as an inhibitor of the calmodulin activation of phosphodiesterase 3′:5′ cyclic nucleotide (PDE), calcineurin has similar effects on adenylate cyclase. Serves as a key enzyme involved in T-cell activation. Also involved in the hyperphosphorylation of tau protein in Alzheimer′s disease and has been shown to prevent calpain-mediated proteolysis of tau in differentiated PC12 cells.

The major calmodulin-binding protein found in the brain. A key enzyme involved in T-cell activation and hyperphosphorylation of tau protein in Alzheimer′s disease.

Physikalische Form

Lyophilized powder containing 0.5% EGTA, buffer salts and stabilizers.

Hinweis zur Analyse

Sigma tests activity in 80 mM Tris, pH 7.5 with 65 mM KCl, 8 mM MgSO₄ and 0.3% albumin.

Sonstige Hinweise

One unit will cause a 50% inhibition of the activated phosphodiesterase, 3′:5′-cyclic nucleotide (P 9529) activity when assayed with two units of activator (P 2277) and 0.1 mM Ca²⁺ in an enzyme coupled system at pH 7.5 at 30 °C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves