Creatinase from microorganisms

Name: Creatinase from microorganisms
Brand: SIGMA

lyophilized powder, ≥4 units/mg solid

Synonym(e):

Creatine Amidinohydrolase

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Über diesen Artikel

CAS-Nummer:

37340-58-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-471-1

MDL number:

MFCD00130875

EG-Nummer:

3.5.3.3 (BRENDA, IUBMB)

Specific activity:

≥4 units/mg solid

Biological source:

bacterial (Actinobacillus spp.)

Technischer Dienst

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Eigenschaften

biological source

bacterial (Actinobacillus spp.)

Quality Segment

200

form

lyophilized powder

specific activity

≥4 units/mg solid

mol wt

~100 kDa

storage temp.

−20°C

Beschreibung

General description

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunits and two defined domains; N and C terminal domains. The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent.

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾²M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu⁺⁺, Hg⁺⁺, Ag⁺
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)

Application

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration.It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

Biochem/physiol Actions

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.