C3172

Kreatininase aus Pseudomonas sp.

lyophilized powder, 100-300 units/mg protein

• Synonym(e): Creatinin-Amidohydrolase
• CAS-Nummer: 9025-13-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-786-8
• MDL number: MFCD00130877
• EG-Nummer: 3.5.2.10 (BRENDA, IUBMB)
• Specific activity: 100-300 units/mg protein

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• specific activity: 100-300 units/mg protein
• mol wt: ~175 kDa
• composition: Protein, 65-85%
• foreign activity: Creatinase and urease ≤1%, Hexokinase and ATPase ≤0.1%
• storage temp.: 2-8°C

Beschreibung

General description

Isoelectric point:4.7
Michaelis constants:3.2 x 10‾²M (Creatinine), 5.7 x 10‾²M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag⁺, Hg⁺⁺, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)

Application

Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine.

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

Biochem/physiol Actions

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

Physical form

Lyophilisiertes Pulver, enthält Saccharose. ~70% des Proteins ist Rinderserumalbumin.

Analysis Note

Proteingehalt mittels Biuret-Reaktion bestimmt.

Other Notes

1 U hydrolysiert 1.0 μmol Creatinin zu Creatin pro Minute bei pH 8.0 und 25 °C

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)