MGAR Human Blood, ECACC 88022014

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., IHW Number: 9014, 88022014

• UNSPSC Code: 41106514
• eCl@ss: 32160903
• Biological source: human blood
• Technique(s): cell culture | mammalian: suitable
• Ethnicity: North American Hispanic
• Gender: female
• Growth mode: Suspension
• Morphology: Lymphoblastoid

Eigenschaften

• Produktname: MGAR human cell line, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., IHW Number: 9014, 88022014
• biological source: human blood
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 88022014-DNA-5UG, pkg of vial of cells 88022014-1VL
• ethnicity: North American Hispanic
• gender: female
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblastoid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: -140 to -196°C

Beschreibung

General description

Homozygous: Yes

Biochem/physiol Actions

A: 2601
Cw: 701
B: 801
HLA DRA: 102
HLA DRB1: 1501
HLA DRB5: 101
HLA DRB6: 201
HLA DQAP: 1.2
HLA DQA1: 1021
HLA DQB1: 602
HLA DPA1: 1
HLA DPB1: 401

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Preparation Note

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

HLA A: 26
HLA A ief: 26.1
HLA C: 7
HLA B: 8
HLA B ief: 8
Bw4/bw6: 6
HLA Dw: 2
HLA DR: 15
HLA DR51: 51
HLA DQ: 6
HLA DP: 4

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Only the genomic DNA extracted from the cell line is available for purchase by commercial organizations, and not the cell line itself. No licensing agreement is required when the DNA extracted from this cell line are used for internal research purposes only. Frozen cell cultures can be supplied only to academic and not-for-profit organisations. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding other authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.