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R0883

RPMI-1640 Medium

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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100 mL
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$16.10
500 mL
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$40.30
1 L
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$66.20
6 x 500 mL
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$209.00
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$356.00
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$756.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217820

$16.10

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Product Name

RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Segment

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
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Application

RPMI-1640 Medium has been used:
  • to culture PC-3 prostate cell line and Jurkat cells
  • to maintain a triple-negative MDA-MB-231 and a luminal type MCF7 human Caucasian breast adenocarcinoma cell line
  • to cultivate circulating tumor cells (CTCs)

Preparation Note

Supplement with 0.3 g/L L-glutamine.

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description

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

Modified, with sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

description

Dutch Modification, with sodium bicarbonate and 20mM HEPES, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

description

Modified, with sodium bicarbonate, without methionine, cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes
HEPES: no

components

phenol red: no
L-glutamine: no
HEPES: no
sodium pyruvate: no
NaHCO3: yes

components

sodium pyruvate: no
HEPES: 20 mM
L-glutamine: no
NaHCO3: yes
phenol red: yes

components

HEPES: no
sodium pyruvate: no
phenol red: yes
L-glutamine: no
NaHCO3: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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Questions

  1. Could you tell me the exact concentration of glucose in this RPMI-1640 medium(cat#R0883 ), please ?

    1 answer

    1. For this RPMI formulation, the glucose concentration is 2 grams per liter.

      Please see the datasheet below which includes the formulations of all classical RPMI-1640 media products:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/229/770/r0883for.pdf

      Helpful?

Reviews

Active Filters

  1. Boston, MA
    • Reviews 2
    • Votes 9
    5 out of 5 stars.

    Great product!

    Reliable cell culture product that gets frequent use

    Helpful?