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CE0050
CelLytic™ MEM Protein Extraction Kit
Synonym(e):
Membrane protein extraction kit
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 513,00 |
Über diesen Artikel
NACRES:
NA.56
UNSPSC Code:
41116134
€ 513,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltentechnique(s)
protein extraction: suitable
Quality Segment
input
mammalian sample(s)
shipped in
wet ice
storage temp.
−20°C
General description
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.
CelLytic™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Application
CelLytic™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
Sufficient reagents supplied for 80 tests.
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
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Dieser Artikel | |||
|---|---|---|---|
| description - | description Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. | description - | description - |
| technique(s) protein extraction: suitable | technique(s) - | technique(s) - | technique(s) protein extraction: suitable |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in ambient |
| input mammalian sample(s) | input mammalian sample(s) | input bacteria and yeast sample(s) | input sample type: mammalian tissue |
| storage temp. −20°C | storage temp. room temp | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Lysis and Separation Buffer 50 mL
- Wash Buffer for CelLytic MEM 50 mL
- Sodium Chloride, 4M Solution 1.5 mL
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB & Preisangaben
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
Lagerklasse
10 - Combustible liquids
Flammpunkt (°F)
188.6 °F - closed cup
Flammpunkt (°C)
87 °C - closed cup
Was ist los?
WGK 2
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2
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