CE0050

CelLytic^™ MEM Protein Extraction Kit

• Synonym(e): Membrane protein extraction kit
• NACRES: NA.56
• UNSPSC Code: 41116134

Eigenschaften

• technique(s): protein extraction: suitable
• Quality Segment: 200
• input: mammalian sample(s)
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

General description

Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.

CelLytic^™ MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.

Application

CelLytic^™ MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.

Sufficient reagents supplied for 80 tests.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS07
• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): 188.6 °F - closed cup
• Flammpunkt (°C): 87 °C - closed cup
• WGK: WGK 2
• Signalwort: Danger
• Gefahrencodes: H315,H319,H412,EUH430
• Sicherheitshinweise: P201 - P202 - P273 - P391 - P405 - P501
• Hazard Classifications: Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2