This kit has not been validated for compatibility with formalin-fixed paraffin-embedded (FFPE) tissue sections. It is designed for cultured cells and advises against using ethanol- or methanol-based fixatives, as these can inactivate the β-galactosidase label critical for senescence detection. FFPE processing typically involves ethanol or reagent-grade alcohol (primarily ethanol with minor methanol and isopropyl alcohol components) during dehydration, which suggests potential incompatibility with the kit’s staining mechanism. Consequently, this kit is likely not suitable for FFPE-processed tissues without protocol optimization, e.g., alternative fixation methods or additional validation to ensure compatibility.
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CS0030
Histochemisches Färbungskit für Seneszenzzellen
sufficient for 100 tests
Synonym(e):
Senescent cells IHC kit, cellular β-galactosidase probe
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Ab HEUTE lieferbarvonSchnelldorf | € 458,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
12352207
€ 458,00
Ab HEUTE lieferbarDetails
Technischer Dienst
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Unterstützung erhaltenusage
sufficient for 100 tests
Quality Segment
packaging
pkg of 1 kit
storage condition
dry at room temperature
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
General description
Das histochemische Färbungskit für seneszente Zellen enthält alle erforderlichen Reagenzien zur Identifizierung von seneszenten Zellen mit einem Assay, der auf histochemischer Färbung zur Bestimmung der β-Galactosidase-Aktivität bei pH-Wert 6 beruht. ββ-Galactosidase-Aktivität ist in seneszenten Zellen nachweisbar, nicht aber in ruhenden, unsterblichen oder Tumorzellen. Zelluläre Seneszenz ist ein Verlauf von Ereignissen, bei denen die Zellen von der Stufe der aktiven Teilung dazu übergehen, sich nicht zu teilen. Der Prozess der zellulären Seneszenz steht mit der Alterung in Verbindung. Der Rückgang der Zellteilung ist nahezu irreversibel und vollständig. In Verbindung mit dem Verlust der Teilungsfähigkeit treten Änderungen der Morphologie, Form und physischen Erscheinung der Zellen sowie deren Genexpressionsmuster auf. Am Ende des Prozesses tritt in der Regel der Zelltod auf, obwohl die Zellen lange Zeit lebensfähig bleiben können.
Application
Das histochemische Färbungskit für Seneszenz-Zellen wird für Folgendes verwendet:
- Seneszenz-assoziierter β-Galactosidase-Assay
- Prüfen auf Seneszenz in humanen Bauchspeicheldrüsenkrebszellen
- Stammzellen aus Fettgewebe (ADSC)
- CBMSC-Zellen
Biochem/physiol Actions
Replikative Seneszenz ist ein Zustand, in dem kein Wachstum stattfindet, und steht in Verbindung mit einem Verlust des Teilungspotenzials, Veränderungen in der Morphologie, Form und physischen Erscheinung der Zelle sowie dem Muster der Genexpression in Zellen.
Legal Information
Hergestellt unter Lizenz gemäß den US-Patenten Nr. 5.491.069 und 5.795.728.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description sufficient for 100 tests | description - | description Cellular Senescence Assay Kits provide all the reagents required to efficiently detect SA-β-gal activity at pH 6.0 in cultured cells & tissue sections. | description Live Cell imaging probe that specifically detects labile ferrous (Fe2+) ions in live cells. |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in - |
| storage condition dry at room temperature | storage condition - | storage condition - | storage condition - |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
| packaging pkg of 1 kit | packaging - | packaging - | packaging - |
| usage sufficient for 100 tests | usage kit sufficient for 100 tests (using a 3.5 cm dish) | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level - |
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
supp_hazards
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Can this kit be used to stain FFPE sections?
1 answer-
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Can we use confocal/basic fluorescent microscope for capturing the expression of Beta Galactosidase after using this staining kit?
1 answer-
This kit has not been tested for use with confocal or fluorescent microscopes. X-gal staining does not involve a specific wavelength and it would be up to the end user to determine suitability. Note below, in a previously submitted question, that this kit has not been tested for dual staining with fluorescent dyes.
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Is there a protocol for staining HCHO-fixed tissue culture cells, specifically for cells growing on coverslips or slides?
1 answer-
For staining purposes, there is no significant difference between staining wells attached to a culture plate or cells attached to a coverslip or glass slide. The primary difference lies in the amount of reagent required for the staining. It is advisable to ensure adequate coverage of the cells, and the insert contains a table that provides guidance on the amount of reagent required for staining.
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Is there a method to extract the stain from cells when using the Senescence Cells Histochemical Staining Kit (Catalog Number CS0030) for cells in transwell inserts where cell counting is not feasible?
1 answer-
The CS0030 kit is intended for cell visualization using a microscope and does not include a specific procedure for dye extraction from cells.
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Does kit CS0030 include a counterstain?
1 answer-
No, the CS0030 kit does not include a counterstain.
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I have been using it well for a long time. thank you I want to stain beta-gal staining and apoptosis-related IF together. Is there a recommended order or method?
1 answer-
The CS0030 kit has not been tested for use in dual staining, it would be up to the end user to determine suitability. There are several factors to consider with regard to attempting this modification.
1. The CS0030 includes a fixation step. Cells are not viable at the time of staining. Therefore any 'live cell' fluorescent stain would not be suitable following fixation.
2. There is an incubation period of 2 hours to overnight. This may deplete or cause certain fluorescent dyes to fade which would mean a 'one-time use' for visualizing the IF-stained cells.
3. The staining mechanism for CS0030 is pH dependent. This may not be suitable for some fluorescent dyes.
4. One possible robust fluorescent stain to consider would be DAPI, which could be applied following the b-gal staining. DAPI will stain fixed cells and can be preserved if stored in the dark.To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further.
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Can Product CS0030, Senescence Cells Histochemical Staining Kit be used with fixed cells/tissues?
1 answer-
Product CS0030, Senescence Cells Histochemical Staining Kit was designed for use with fresh (not-fixed) cells. The kit contains a fixation buffer to fix the cells. It is 10% formaldehyde, 1% glutaraldehyde in DPBS at 1X. The cells will be fixed prior to staining. The staining will show the beta-galactosidase activity that was present at the time of fixation.We tested the kit only on cells and we have no experience with tissue sections. The following papers use an assay for ßGAL on tissue sections which suggests that the kit will work with tissue sections.Castro P, et al., Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia. Prostate, 60(2), 153-159 (2004).Chen, Z., et al., Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature, 436, 725-730 (2005).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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