This product is supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON™ X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8. For assay purposes, the product is diluted in 50 mM MOP HCl Buffer, pH 7.4. Other published literature describes dilution to 1 U/mL using 20 mM sodium phosphate buffer containing 1 mM magnesium chloride (pH 7.2).
Fortfahren mit
K1502
α-Ketoglutarate Dehydrogenase from porcine heart
buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry)
Synonym(e):
Multienzyme 2-oxoglutarate dehydrogenase complex
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
20 units | Warenkorb auf Verfügbarkeit prüfen | € 213,00 |
50 units | Warenkorb auf Verfügbarkeit prüfen | € 447,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EG-Nummer:
MDL number:
Specific activity:
0.1-1.0 units/mg protein (Lowry)
Technischer Dienst
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Unterstützung erhaltenform
buffered aqueous glycerol solution
Quality Segment
specific activity
0.1-1.0 units/mg protein (Lowry)
foreign activity
pyruvate dehydrogenase ≤20%
shipped in
dry ice
storage temp.
−20°C
General description
Research Area: Neuroscience
α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).
α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).
Application
α-Ketoglutarate Dehydrogenase from the porcine heart has been used:
- to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
- to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
- as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons
Biochem/physiol Actions
α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.
α-Ketoglutarate dehydrogenase is most responsive to alterations in the tumor microenvironment and contributes to the adaptive metabolic response in cancer. Inhibiting α-ketoglutarate dehydrogenase counteracts lung metastasis associated with breast cancer.
Physical form
Supplied as a 50% glycerol solution containing ~9 mg per mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3% TRITON™ X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8.
Analysis Note
May contain traces of polyethylene glycol.
Other Notes
One unit will convert 1.0 μmole of β-NAD to β-NADH per min at pH 7.4 at 30 °C in the presence of saturating levels of coenzyme A.
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Dieser Artikel | |||
|---|---|---|---|
| description buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry) | description buffered aqueous glycerol solution, 0.1-1.0 units/mg protein (Lowry) | description buffered aqueous glycerol solution, ≥600 units/mg protein (biuret) | description Type I, 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart) |
| specific activity 0.1-1.0 units/mg protein (Lowry) | specific activity 0.1-1.0 units/mg protein (Lowry) | specific activity 600-1000 units/mg protein (biuret) | specific activity 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart) |
| form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in - |
| foreign activity pyruvate dehydrogenase ≤20% | foreign activity α-ketoglutarate dehydrogenase ≤15% | foreign activity Glutamic-Pyruvic Transaminase ≤0.01%, Glutamic-Oxalacetic Transaminase ≤0.01% | foreign activity - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Lagerklasse
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
9002-93-1
CAS No.
9002-93-1
CAS No.
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I would like to ask about the recommended solvent or buffer for diluting α-Ketoglutarate dehydrogenase (Product No. K1502). Could you please advise which solvent or buffer is recommended for diluting this enzyme before use?
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